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Landscape and Dynamics of Single Immune Cells in Hepatocellular Carcinoma.
Cell ( IF 45.5 ) Pub Date : 2019-10-31 , DOI: 10.1016/j.cell.2019.10.003 Qiming Zhang 1 , Yao He 2 , Nan Luo 3 , Shashank J Patel 4 , Yanjie Han 1 , Ranran Gao 1 , Madhura Modak 5 , Sebastian Carotta 6 , Christian Haslinger 7 , David Kind 7 , Gregory W Peet 4 , Guojie Zhong 1 , Shuangjia Lu 1 , Weihua Zhu 8 , Yilei Mao 9 , Mengmeng Xiao 10 , Michael Bergmann 11 , Xueda Hu 1 , Sid P Kerkar 4 , Anne B Vogt 12 , Stefan Pflanz 5 , Kang Liu 4 , Jirun Peng 3 , Xianwen Ren 1 , Zemin Zhang 13
Cell ( IF 45.5 ) Pub Date : 2019-10-31 , DOI: 10.1016/j.cell.2019.10.003 Qiming Zhang 1 , Yao He 2 , Nan Luo 3 , Shashank J Patel 4 , Yanjie Han 1 , Ranran Gao 1 , Madhura Modak 5 , Sebastian Carotta 6 , Christian Haslinger 7 , David Kind 7 , Gregory W Peet 4 , Guojie Zhong 1 , Shuangjia Lu 1 , Weihua Zhu 8 , Yilei Mao 9 , Mengmeng Xiao 10 , Michael Bergmann 11 , Xueda Hu 1 , Sid P Kerkar 4 , Anne B Vogt 12 , Stefan Pflanz 5 , Kang Liu 4 , Jirun Peng 3 , Xianwen Ren 1 , Zemin Zhang 13
Affiliation
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The immune microenvironment of hepatocellular carcinoma (HCC) is poorly characterized. Combining two single-cell RNA sequencing technologies, we produced transcriptomes of CD45+ immune cells for HCC patients from five immune-relevant sites: tumor, adjacent liver, hepatic lymph node (LN), blood, and ascites. A cluster of LAMP3+ dendritic cells (DCs) appeared to be the mature form of conventional DCs and possessed the potential to migrate from tumors to LNs. LAMP3+ DCs also expressed diverse immune-relevant ligands and exhibited potential to regulate multiple subtypes of lymphocytes. Of the macrophages in tumors that exhibited distinct transcriptional states, tumor-associated macrophages (TAMs) were associated with poor prognosis, and we established the inflammatory role of SLC40A1 and GPNMB in these cells. Further, myeloid and lymphoid cells in ascites were predominantly linked to tumor and blood origins, respectively. The dynamic properties of diverse CD45+ cell types revealed by this study add new dimensions to the immune landscape of HCC.
中文翻译：
肝细胞癌中单个免疫细胞的景观和动态。
肝细胞癌（HCC）的免疫微环境尚不清楚。结合两种单细胞 RNA 测序技术，我们从五个免疫相关部位生成了 HCC 患者的 CD45+ 免疫细胞的转录组：肿瘤、邻近肝脏、肝淋巴结 (LN)、血液和腹水。 LAMP3+ 树突状细胞 (DC) 簇似乎是传统 DC 的成熟形式，并且具有从肿瘤迁移到 LN 的潜力。 LAMP3+ DC 还表达多种免疫相关配体，并表现出调节淋巴细胞多种亚型的潜力。在肿瘤中表现出不同转录状态的巨噬细胞中，肿瘤相关巨噬细胞 (TAM) 与不良预后相关，我们确定了 SLC40A1 和 GPNMB 在这些细胞中的炎症作用。此外，腹水中的骨髓细胞和淋巴细胞主要分别与肿瘤和血液来源有关。这项研究揭示的不同 CD45+ 细胞类型的动态特性为 HCC 的免疫景观增添了新的维度。
更新日期：2019-11-09
中文翻译：
肝细胞癌中单个免疫细胞的景观和动态。
肝细胞癌（HCC）的免疫微环境尚不清楚。结合两种单细胞 RNA 测序技术，我们从五个免疫相关部位生成了 HCC 患者的 CD45+ 免疫细胞的转录组：肿瘤、邻近肝脏、肝淋巴结 (LN)、血液和腹水。 LAMP3+ 树突状细胞 (DC) 簇似乎是传统 DC 的成熟形式，并且具有从肿瘤迁移到 LN 的潜力。 LAMP3+ DC 还表达多种免疫相关配体，并表现出调节淋巴细胞多种亚型的潜力。在肿瘤中表现出不同转录状态的巨噬细胞中，肿瘤相关巨噬细胞 (TAM) 与不良预后相关，我们确定了 SLC40A1 和 GPNMB 在这些细胞中的炎症作用。此外，腹水中的骨髓细胞和淋巴细胞主要分别与肿瘤和血液来源有关。这项研究揭示的不同 CD45+ 细胞类型的动态特性为 HCC 的免疫景观增添了新的维度。
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