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A clinical-grade acellular matrix for esophageal replacement.
Journal of Tissue Engineering and Regenerative Medicine ( IF 3.1 ) Pub Date : 2019-11-07 , DOI: 10.1002/term.2983
Lousineh Arakelian 1, 2 , Clémentine Caille 1, 2 , Lionel Faivre 1, 2 , Laurent Corté 3, 4 , Patrick Bruneval 5 , Sara Shamdani 6 , Camille Flageollet 6 , Patricia Albanese 6 , Thomas Domet 1, 2 , Mohamed Jarraya 7 , Niclas Setterblad 8 , Sabrina Kellouche 9 , Jérôme Larghero 1, 2 , Pierre Cattan 2, 10 , Valérie Vanneaux 1, 2
Affiliation  

In pathologies of the esophagus such as esophageal atresia, cancers, and caustic injuries, methods for full thickness esophageal replacement require the sacrifice of healthy intra-abdominal organs such as the stomach and the colon and are associated with high morbidity, mortality, and poor functional results. To overcome these problems, tissue engineering methods are developed to create a substitute with scaffolds and cells. The aim of this study was to develop a simple and safe decellularization process in order to obtain a clinical grade esophageal extracellular matrix. Following the decontamination step, porcine esophagi were decellularized in a bioreactor with sodium dodecyl sulfate and ethylenediaminetetraacetic acid for 3 days and were rinsed with deionized water. DNA was eliminated by a 3-hr DNase treatment. To remove any residual detergent, the matrix was then incubated with an absorbing resin. The resulting porcine esophageal matrix was characterized by the assessment of the efficiency of the decellularization process (DNA quantification), evaluation of sterility and absence of cytotoxicity, and its composition and biomechanical properties, as well as the possibility to be reseeded with mesenchymal stem cells. Complete decellularization with the preservation of the general structure, composition, and biomechanical properties of the native esophageal matrix was obtained. Sterility was maintained throughout the process, and the matrix showed no cytotoxicity. The resulting matrix met clinical grade criteria and was successfully reseeded with mesenchymal stem cells..

中文翻译:

用于食道置换的临床级脱细胞基质。

在食道病变(例如食道闭锁,癌症和苛性损伤)中,全厚度食道置换方法需要牺牲健康的腹腔内器官(如胃和结肠),并伴有高发病率,死亡率和功能差结果。为了克服这些问题,开发了组织工程方法以产生支架和细胞的替代物。这项研究的目的是开发一种简单安全的脱细胞方法,以获得临床级的食道细胞外基质。净化步骤之后,将猪食管在生物反应器中用十二烷基硫酸钠和乙二胺四乙酸脱细胞3天,并用去离子水冲洗。通过3小时的DNase处理消除了DNA。要去除任何残留的清洁剂,然后将基质与吸收性树脂一起孵育。所得猪食道基质的特征在于脱细胞过程效率的评估(DNA定量),无菌性和无细胞毒性的评估,其组成和生物力学特性以及间充质干细胞再播种的可能性。获得了完整的脱细胞作用,并保留了天然食管基质的一般结构,组成和生物力学特性。在整个过程中保持无菌状态,并且基质没有细胞毒性。所得基质符合临床等级标准,并成功地与间充质干细胞一起接种。所得猪食道基质的特征在于脱细胞过程效率的评估(DNA定量),无菌性和无细胞毒性的评估,其组成和生物力学特性以及间充质干细胞再播种的可能性。获得了完整的脱细胞作用,并保留了天然食管基质的一般结构,组成和生物力学特性。在整个过程中保持无菌状态,并且基质没有细胞毒性。所得基质符合临床等级标准,并成功地与间充质干细胞一起接种。所得猪食道基质的特征在于脱细胞过程效率的评估(DNA定量),无菌性和无细胞毒性的评估,其组成和生物力学特性以及间充质干细胞再播种的可能性。获得了完整的脱细胞作用,并保留了天然食管基质的一般结构,组成和生物力学特性。在整个过程中保持无菌状态,并且基质没有细胞毒性。所得基质符合临床等级标准,并成功地与间充质干细胞一起接种。其组成和生物力学特性,以及与间充质干细胞一起播种的可能性。获得了完整的脱细胞作用,并保留了天然食管基质的一般结构,组成和生物力学特性。在整个过程中保持无菌状态,并且基质没有细胞毒性。所得基质符合临床等级标准,并成功地与间充质干细胞一起接种。其组成和生物力学特性,以及与间充质干细胞一起播种的可能性。获得了完整的脱细胞作用,并保留了天然食管基质的一般结构,组成和生物力学特性。在整个过程中保持无菌状态,并且基质没有细胞毒性。所得基质符合临床等级标准,并成功地与间充质干细胞一起播种。
更新日期:2019-11-08
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