A new cyclic lipopeptide (CLP) orfamide H (1) was purified and identified from the cultural broth of the bacterial strain Pseudomonas protegens CHA0. The crude extract of the strain CHA0 was obtained by an acid-aided precipitation process, then the compound 1 was purified by reversed-phase high-performance liquid chromatography (RP-HPLC). Subsequently, the chemical structure of orfamide H was determined by 1D and 2D nuclear magnetic resonance (NMR) and mass spectrometry (MS). Further biological assays indicate that the new CLP orfamide H shows the activity on inhibiting the appressoria formation of the fungus Magnaporthe oryzae, the causal agent of the blast disease in rice. Taken all together, these results indicated that the new CLP orfamide H has the capacity to be developed as an agrichemical to control blast disease in rice.

中文翻译：

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蛋白质假单胞菌CHA0中新的环状脂肽orfamide H的分离和表征。

从细菌菌株假单胞菌蛋白CHA0的培养液中纯化并鉴定了一种新的环脂肽（CLP）或甲酰胺H（1）。通过酸辅助沉淀法获得CHA0菌株的粗提取物，然后通过反相高效液相色谱法（RP-HPLC）纯化化合物1。随后，通过1D和2D核磁共振（NMR）和质谱（MS）确定了Orfamide H的化学结构。进一步的生物学分析表明，新的CLP orfamide H表现出抑制稻瘟病的病原体真菌Magnaporthe oryzae的感官形成的活性。综上所述，这些结果表明，新的CLP orfamide H具有开发成为控制水稻稻瘟病的农业化学品的能力。