1. Bats are becoming increasingly important as an experimental model due to their unique biological features. These include the ability of powered flight, minimal consequences from a heightened metabolic state, extended longevity in most species and minimal inflammation in response to most otherwise pathogenic viruses. To date there has been limited work done on the optimal procedures for necropsy, extraction of tissues or preparation of cell suspensions for downstream experimental work.
2. Here we use Pteropus alecto black flying fox as an example model of the fruit bat to develop optimal procedures for anaesthetizing, necropsy methods, safety, sequence and protocols for cell/tissue extraction and isolation protocols.
3. These methods were successfully used to yield high‐quality RNA, DNA and protein samples from tissues along with viable cells for various molecular and immunological studies. Procedures utilized are suitable for comparative biology studies with most protocols being directly modified from those used in mice and humans. While mainly optimized for the larger fruit bats (flying foxes) in this study, the majority of protocols can readily be adapted to all species of bats.
4. This study provides the framework for greater consistency with in vivo bat experiments, application for comparative biology studies and greater engagement of the bat community for suitable protocols to be harmoniously adopted.

中文翻译：

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优化节食蝙蝠的解剖，样品收集和细胞分离方案

1. 由于其独特的生物学特性，蝙蝠作为实验模型正变得越来越重要。这些包括动力飞行的能力，新陈代谢状态增强带来的最小后果，大多数物种的寿命延长以及对大多数原本具有致病性的病毒的响应最小的炎症。迄今为止，在尸检，组织提取或制备用于下游实验工作的细胞悬液的最佳程序方面，所做的工作还很有限。
2. 在这里，我们使用翼手龙（Pteropus alecto）黑狐作为果蝠的示例模型，以开发用于麻醉，尸检方法，安全性，序列和实验方案的最佳程序，以用于细胞/组织提取和分离实验方案。
3. 这些方法已成功用于从组织以及活细胞中获得高质量的RNA，DNA和蛋白质样品，用于各种分子和免疫学研究。所使用的程序适用于比较生物学研究，其中大多数规程可以直接从小鼠和人类中使用的规程中进行修改。尽管在本研究中主要针对较大的果蝠（果蝠）进行了优化，但大多数协议都可以轻松适应所有种类的蝙蝠。
4. 这项研究提供了与体内蝙蝠实验更大一致性，进行比较生物学研究的应用以及蝙蝠群落更多地参与以和谐地采用适当方案的框架。