We report on wide‐field time‐correlated single photon counting (TCSPC)‐based fluorescence lifetime imaging microscopy (FLIM) with lightsheet illumination. A pulsed diode laser is used for excitation, and a crossed delay line anode image intensifier, effectively a single‐photon sensitive camera, is used to record the position and arrival time of the photons with picosecond time resolution, combining low illumination intensity of microwatts with wide‐field data collection. We pair this detector with the lightsheet illumination technique, and apply it to 3D FLIM imaging of dye gradients in human cancer cell spheroids, and C. elegans.

中文翻译：

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具有宽视场时间相关单光子计数的光片荧光寿命成像显微镜。


我们报告了具有光片照明的基于宽视场时间相关单光子计数（TCSPC）的荧光寿命成像显微镜（FLIM）。脉冲二极管激光器用于激发，交叉延迟线阳极图像增强器（实际上是单光子敏感相机）用于以皮秒时间分辨率记录光子的位置和到达时间，将微瓦的低照度与广域数据采集。我们将该探测器与光片照明技术配对，并将其应用于人类癌细胞球体和线虫中染料梯度的 3D FLIM 成像。