The study is devoted to the oxidative modification of immunoglobulin G (IgG) on the surface of peroxidase-like iron oxide magnetic nanoparticles (MNPs) under conditions of induced reactive oxygen species (ROS) generation and without them. A pronounced change of thermodynamic parameters of denaturation has been detected for IgG in solutions containing MNPs under hydrogen peroxide action during 24 h of incubation. Dynamic light scattering measurements and UV-Visible spectrophotometry have been used to show aggregation in these solutions. Ferromagnetic resonance (FMR) was used to compare IgG coating thickness on individual MNPs under conditions of induced ROS generation and without them. The similarity between IgG adsorption on MNPs under these conditions after 24 h of incubation has been confirmed by the fluorescence measurements. The sites of IgG oxidative modifications that take place on MNPs surface and some evidences of the influence of oxidative modification and adsorption on the chemical structure of IgG were revealed by HPLC MS/MS analysis.

中文翻译：

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免疫球蛋白G与过氧化物酶样氧化铁纳米颗粒之间的相互作用：蛋白质的物理化学和结构特征。

这项研究致力于在诱导型活性氧（ROS）生成的条件下和没有它们的条件下，过氧化物酶样氧化铁磁性纳米颗粒（MNPs）表面上免疫球蛋白G（IgG）的氧化修饰。在孵育24小时的过程中，在过氧化氢作用下，含有MNP的溶液中的IgG已检测到变性的热力学参数发生了显着变化。动态光散射测量和紫外可见分光光度法已用于显示这些溶液中的聚集。使用铁磁共振（FMR）来比较在诱导的ROS生成条件下和没有它们的条件下单个MNP上IgG的涂层厚度。孵育24小时后，在这些条件下，在这些条件下IgG吸附在MNP上的相似性已通过荧光测量得到证实。