BACKGROUND The objective of this pilot study was to assess iron (Fe), copper (Cu), zinc (Zn), and manganese (Mn) status (hair, serum, and urine) and speciation (serum) in Parkinson's disease (PD) patients. METHODS A pilot study involving a total of 27 subjects (13 PD patients, 14 controls) was performed. Serum, urine, and hair metal content was assessed using ICP-MS. Speciation analysis of Cu, Zn, Fe, and Mn was performed using a hybrid HPLC-ICP-MS system. RESULTS Group comparisons did not reveal any significant group difference in serum Cu, Zn, Fe, and Mn total metal level between PD patients and controls. Speciation analysis revealed a significant decrease in Cu/ceruloplasmin copper in association with elevation of low-molecular weight species (amino acids)-bound copper. It is proposed that in PD, binding of Cu(II) ions to ceruloplasmin is reduced and free copper ions coordinate with low molecular weight ligands. The level of Mn-albumin complexes in PD patients was more than 4-fold higher as compared to the respective value in the control group. The observed difference may be considered as a marker of redistribution between high and low molecular weight ligands. CONCLUSIONS Metal speciation is significantly affected in serum of PD-patients. These findings are indicative of the potential role of metal metabolism and PD pathogenesis, although the exact mechanisms of such associations require further detailed studies.

中文翻译：

---

帕金森氏病患者铜，铁，锌和锰状况和形态的评估：一项试点研究。

背景这项初步研究的目的是评估帕金森病（PD）中铁（Fe），铜（Cu），锌（Zn）和锰（Mn）的状态（头发，血清和尿液）和形态（血清）耐心。方法进行了一项涉及27位受试者（13位PD患者，14位对照）的试验研究。使用ICP-MS评估血清，尿液和头发中的金属含量。使用混合型HPLC-ICP-MS系统对Cu，Zn，Fe和Mn进行形态分析。结果组间比较未发现PD患者与对照组之间血清Cu，Zn，Fe和Mn总金属水平有任何显着的组间差异。形态分析显示，与低分子量物质（氨基酸）结合的铜升高，Cu /铜蓝蛋白铜显着减少。建议在PD中，Cu（II）离子与铜蓝蛋白的结合减少，游离铜离子与低分子量配体配位。与对照组相比，PD患者中的锰-白蛋白复合物水平高出4倍以上。观察到的差异可以视为高分子量和低分子量配体之间重新分布的标志。结论PD患者的血清中金属形态受到显着影响。这些发现表明金属代谢和PD发病机制的潜在作用，尽管这种关联的确切机制需要进一步的详细研究。观察到的差异可以视为高分子量和低分子量配体之间重新分布的标志。结论PD患者的血清中金属形态受到显着影响。这些发现表明金属代谢和PD发病机制的潜在作用，尽管这种关联的确切机制需要进一步的详细研究。观察到的差异可以视为高分子量和低分子量配体之间重新分布的标志。结论PD患者的血清中金属形态受到显着影响。这些发现表明金属代谢和PD发病机制的潜在作用，尽管这种关联的确切机制需要进一步的详细研究。