Two pale green mutants of the green alga Chlamydomonas reinhardtii, which have been used over the years in many photosynthesis studies, the BF4 and p71 mutants, were characterized and their mutated gene identified in the nuclear genome. The BF4 mutant is defective in the insertase Alb3.1 whereas p71 is defective in cpSRP43. The two mutants showed strikingly similar deficiencies in most of the peripheral antenna proteins associated with either photosystem I or photosystem 2. As a result the two photosystems have a reduced antenna size with photosystem 2 being the most affected. Still up to 20% of the antenna proteins remain in these strains, with the heterodimer Lhca5/Lhca6 showing a lower sensitivity to these mutations. We discuss these phenotypes in light of those of other allelic mutants that have been described in the literature and suggest that eventhough the cpSRP route serves as the main biogenesis pathway for antenna proteins, there should be an escape pathway which remains to be genetically identified.

中文翻译：

---

莱茵衣藻的BF4和p71天线突变体。

多年以来在许多光合作用研究中使用的两个绿藻衣藻衣藻的淡绿色突变体BF4和p71突变体的特征在于它们的突变基因，并在核基因组中鉴定出它们的突变基因。BF4突变体在插入酶Alb3.1中有缺陷，而p71在cpSRP43中有缺陷。这两个突变体在与光系统I或光系统2相关的大多数外围天线蛋白中显示出惊人的相似缺陷。结果，这两个光系统的天线尺寸减小了，其中光系统2受的影响最大。仍然有高达20％的触角蛋白保留在这些菌株中，异二聚体Lhca5 / Lhca6对这些突变的敏感性较低。