Analysis of luteinizing hormone (LH): Validation of a commercial ELISA kit for LH analysis and quantification in doping control samples.,Drug Testing and Analysis

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Analysis of luteinizing hormone (LH): Validation of a commercial ELISA kit for LH analysis and quantification in doping control samples.
Drug Testing and Analysis ( IF 2.6 ) Pub Date : 2020-01-10 , DOI: 10.1002/dta.2716
Riika Mäkelä ₁ , Antti Leinonen ₁ , Tina Suominen ₁

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Luteinizing hormone (LH) is a dimeric glycoprotein produced and secreted by the pituitary gland, with a molecular weight of approximately 30 000 Da. The main clinical use for exogenous LH‐administration is typically linked to the treatment of infertility, in both men and women. The desired effect of LH misuse in sport is due to the enhancement of testicular production of testosterone. Elevated LH levels may also indicate the usage of gonadotropin‐releasing factors or estrogen blockers. Therefore, LH is listed by the World Anti‐Doping Agency (WADA) as a prohibited substance in male athletes, and according to the WADA technical document, laboratories should determine the the total LH concentrations in urine. The TD lists two different assays that are suitable for measuring total LH in urine, Delfia and Siemens Immulite. Other fit‐for‐purpose assays can be used, as long as they are capable of detecting total LH in urine. In case an assay not listed in the TD will be used, population‐based reference values have to be determined in the validation procedure.

中文翻译：

黄体生成激素（LH）分析：验证用于掺杂对照样品中LH分析和定量的商业ELISA试剂盒。

黄体生成激素（LH）是一种由垂体产生和分泌的二聚体糖蛋白，分子量约为30 000 Da。在男性和女性中，外源性LH给药的主要临床用途通常与不育症的治疗有关。运动中LH滥用的理想效果归因于睾丸激素睾丸激素水平的提高。LH水平升高也可能表明使用促性腺激素释放因子或雌激素阻滞剂。因此，LH被世界反兴奋剂机构（WADA）列为男性运动员中的违禁物质，根据WADA技术文件，实验室应确定尿液中LH的总浓度。TD列出了两种适用于测量尿液中总LH的测定法：Delfia和Siemens Immulite。只要能够检测尿液中的总LH，也可以使用其他适合目的的测定法。如果要使用未在TD中列出的检测方法，则必须在验证程序中确定基于人群的参考值。

更新日期：2020-01-10

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