Endogenous estrogens play major roles in many aspects of female reproductive development in fish. In order to develop a relatively high-throughput assay to determine the potential impact on reproductive development, vitellogenic rainbow trout ovarian follicles were exposed to a suite of contaminants in vitro and then assessed for the ability to produce estradiol-17β (E2) after a 500 ng/ml salmon gonadotropin (sGTH) challenge. There was a positive correlation between ovarian follicle size and E2 production, but an inverse correlation between size and responsiveness to sGTH. Significant impacts on E2 levels were observed following treatment with different endocrine disrupting chemicals, such as 17α-ethinylestradiol (EE2), prochloraz, or trenbolone. EE2 was remarkably potent and significantly reduced ovarian follicle responsiveness to sGTH at concentrations as low as 0.1 nM. Of the other contaminants tested, only tamoxifen impacted E2 levels, and only at concentrations near the limits of solubility. Flutamide, fluoxetine, 4-hydroxy tamoxifen, hydroxyflutamide, and norfluoxetine had little or no impact. Quantitative PCR analyses of steroidogenesis-related genes were carried out on EE2 treated ovarian follicles, but significant transcriptional responses to EE2 were not observed. Overall, this study suggests that xenoestrogens and anti-estrogens are more likely to interfere with ovarian E2 synthesis than other classes of EDCs. This also provides a template for further testing of the effects of EDCs on ovarian function.

中文翻译：

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卵黄形成的虹鳟鱼卵泡在体外暴露于内分泌干扰化学物质会改变基础雌二醇17β的产生和对促性腺激素激发的反应性。

内源性雌激素在鱼类雌性生殖发育的许多方面起着重要作用。为了开发相对较高通量的测定方法以确定对生殖发育的潜在影响，将卵黄形成的虹鳟卵巢卵泡在体外暴露于一系列污染物，然后在500℃后评估其产生雌二醇17β（E2）的能力。 ng / ml鲑鱼促性腺激素（sGTH）攻击。卵巢卵泡大小与E2产生呈正相关，但大小与对sGTH的反应性呈反相关。在使用不同的内分泌干扰化学物质（例如17α-炔雌醇（EE2），丙草胺或群勃龙）处理后，观察到对E2水平的显着影响。在低至0.1 nM的浓度下，EE2非常有效，并显着降低了卵巢对sGTH的响应。在测试的其他污染物中，只​​有他莫昔芬会影响E2水平，并且仅在接近溶解度极限的浓度下会影响E2水平。氟他米特，氟西汀，4-羟基他莫昔芬，羟基氟他胺和去氟西汀几乎没有影响。在EE2处理过的卵泡上进行了类固醇生成相关基因的定量PCR分析，但未观察到对EE2的明显转录反应。总体而言，这项研究表明异种雌激素和抗雌激素比其他类别的EDC更可能干扰卵巢E2的合成。这也提供了进一步测试EDC对卵巢功能的影响的模板。只有他莫昔芬会影响E2水平，并且仅在接近溶解度极限的浓度下才会影响。氟他胺，氟西汀，4-羟基他莫昔芬，羟基氟他胺和去氟西汀几乎没有影响。在EE2处理过的卵泡上进行了类固醇生成相关基因的定量PCR分析，但未观察到对EE2的明显转录反应。总体而言，这项研究表明异种雌激素和抗雌激素比其他类别的EDC更可能干扰卵巢E2的合成。这也提供了一个模板，用于进一步测试EDC对卵巢功能的影响。只有他莫昔芬会影响E2水平，并且仅在接近溶解度极限的浓度下才会影响E2水平。氟他胺，氟西汀，4-羟基他莫昔芬，羟基氟他胺和去氟西汀几乎没有影响。在EE2处理过的卵泡上进行了类固醇生成相关基因的定量PCR分析，但未观察到对EE2的明显转录反应。总体而言，这项研究表明异种雌激素和抗雌激素比其他类别的EDC更可能干扰卵巢E2的合成。这也提供了一个模板，用于进一步测试EDC对卵巢功能的影响。在EE2处理过的卵泡上进行了类固醇生成相关基因的定量PCR分析，但未观察到对EE2的明显转录反应。总体而言，这项研究表明异种雌激素和抗雌激素比其他类别的EDC更可能干扰卵巢E2的合成。这也提供了一个模板，用于进一步测试EDC对卵巢功能的影响。在EE2处理的卵泡上进行了类固醇生成相关基因的定量PCR分析，但未观察到对EE2的明显转录反应。总体而言，这项研究表明异种雌激素和抗雌激素比其他类别的EDC更可能干扰卵巢E2的合成。这也提供了一个模板，用于进一步测试EDC对卵巢功能的影响。