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Changes in enzyme activities and amino acids and their relations with phenolic compounds contents in okra treated by LED lights of different colors
Food and Bioprocess Technology ( IF 5.3 ) Pub Date : 2019-10-25 , DOI: 10.1007/s11947-019-02359-y
Nattanan Wilawan , Luxsika Ngamwonglumlert , Sakamon Devahastin , Naphaporn Chiewchan

Light-emitting diodes (LEDs) of different wavelengths or colors (i.e., white, red, blue, and green) were used to treat postharvest okra, which is a rich source of phenolic compounds. Relationships between changes in the activities of key enzymes involving in the formation of phenolics (i.e., 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, chorismate mutase, anthranilate synthase, and phenylalanine ammonia lyase) and their contents upon different LED light treatments were for the first time investigated and are fully discussed. The contents of three intermediate amino acids (i.e., phenylalanine, tyrosine, and tryptophan) that formed during light treatments were also measured to confirm the enzyme activities data. White and blue light treatments increased the content of phenolics in the treated okra, while red and green lights increased the formation of other compounds. These results could be well explained by the changing levels of the measured enzyme activities and amino acids contents.



中文翻译:

不同颜色LED灯处理秋葵中酶活性和氨基酸的变化及其与酚类化合物含量的关系

不同波长或颜色(即白色,红色,蓝色和绿色)的发光二极管(LED)用于治疗收获后的秋葵,秋葵是酚类化合物的丰富来源。在不同LED灯下,涉及酚类形成的关键酶活性变化(即3-脱氧-D-阿拉伯-庚酸七磷酸合酶,分支酸突变,邻氨基苯甲酸合酶和苯丙氨酸氨裂合酶)之间的关系首次对治疗方法进行了研究,并进行了充分讨论。还测量了在光处理过程中形成的三个中间氨基酸(即苯丙氨酸,酪氨酸和色氨酸)的含量,以确认酶活性数据。白光和蓝光处理增加了处理过的秋葵中酚类的含量,而红色和绿色的光会增加其他化合物的形成。这些结果可以通过测量的酶活性和氨基酸含量的变化水平很好地解释。

更新日期:2019-10-25
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