AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration.,The Plant Cell

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AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration.
The Plant Cell ( IF 10.0 ) Pub Date : 2019-10-24 , DOI: 10.1105/tpc.19.00378
Bin-Bin Ye _{1,

2} , Guan-Dong Shang _{1,

2} , Yu Pan ₃ , Zhou-Geng Xu _{1,

2} , Chuan-Miao Zhou ₁ , Ying-Bo Mao ₄ , Ning Bao ₅ , Lijun Sun ₃ , Tongda Xu ₆ , Jia-Wei Wang _{7,

8}

Affiliation

National Key Laboratory of Plant Molecular Genetics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, 200032 Shanghai, P. R. China.
University of Chinese Academy of Sciences, 200032 Shanghai, P. R. China.
School of Life Sciences, Nantong University, Nantong, 226019 Jiangsu, P. R. China.
Chinese Academy of Sciences Key Laboratory of Insect Developmental and Evolutionary Biology, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, 200032 Shanghai, P. R. China.
School of Public Health, Nantong University, Nantong, 226019 Jiangsu, P. R. China.
Fujian Agriculture and Forestry University-University of California Riverside Joint Center, Horticulture Biology and Metabolomics Center, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, 350002 Fuzhou, P. R. China.
National Key Laboratory of Plant Molecular Genetics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, 200032 Shanghai, P. R. China
ShanghaiTech University, Shanghai 200031, P. R. China.

Age and wounding are two major determinants for regeneration. In plants, the root regeneration is triggered by wound-induced auxin biosynthesis. As plants age, the root regenerative capacity gradually decreases. How wounding leads to the auxin burst and how age and wound signals collaboratively regulate root regenerative capacity are poorly understood. Here, we show that the increased levels of three closely-related miR156-targeted Arabidopsis (Arabidopsis thaliana) SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, SPL2, SPL10, and SPL11, suppress root regeneration with age by inhibiting wound-induced auxin biosynthesis. Mechanistically, we find that a subset of APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factors including ABSCISIC ACID REPRESSOR1 and ERF109 is rapidly induced by wounding and serves as a proxy for wound signal to induce auxin biosynthesis. In older plants, SPL2/10/11 directly bind to the promoters of AP2/ERFs and attenuates their induction, thereby dampening auxin accumulation at the wound. Our results thus identify AP2/ERFs as a hub for integration of age and wound signal for root regeneration.

中文翻译：

AP2/ERF 转录因子整合年龄和伤口信号以促进根再生。

年龄和受伤是再生的两个主要决定因素。在植物中，根再生是由伤口诱导的生长素生物合成触发的。随着植物年龄的增长，根系的再生能力逐渐下降。受伤如何导致生长素爆发，以及年龄和伤口信号如何协同调节根部再生能力，人们知之甚少。在这里，我们发现三种密切相关的 miR156 靶向拟南芥 SQUAMOSA 启动子结合蛋白样 (SPL) 转录因子 SPL2、SPL10 和 SPL11 的水平增加，通过抑制伤口诱导的根再生，抑制根再生。生长素生物合成。从机制上讲，我们发现 APETALA2/乙烯反应因子 (AP2/ERF) 转录因子的子集（包括脱落酸抑制因子 1 和 ERF109）会被伤口快速诱导，并作为伤口信号的代表来诱导生长素生物合成。在较老的植物中，SPL2/10/11 直接与 AP2/ERF 的启动子结合并减弱它们的诱导，从而抑制伤口处生长素的积累。因此，我们的结果确定 AP2/ERF 是整合年龄和伤口信号以促进根再生的枢纽。

更新日期：2020-01-10

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