The rapid advancement of bio-orthogonal click chemistry in the past decade has enabled the study and precise manipulation of biological processes within living organisms. The capability to induce fast and selective chemical reactions between two exogenous complementary moieties in living systems, with negligible perturbation to their native activities, have rendered bio-orthogonal click chemistry highly promising for a multitude of bioapplications. This review provides an overview of recent developments in bio-orthogonal click chemistry for bioimaging in living organisms. Bio-orthogonal click reactions commonly performed in in vivo systems and their in vivo biological labeling and imaging applications, particularly for the visualization of biomolecular processes, therapeutic cells, tumors, and bacteria, are discussed. Potential future directions of this exciting area are also presented.

在过去的十年中，生物正交点击化学的快速发展使人们能够研究和精确控制生物体内的生物过程。能够在生命系统中的两个外源互补部分之间诱导快速而选择性的化学反应的能力，而对其天然活性的影响却微不足道，这使得生物正交点击化学在众多生物应用中具有很高的前景。这篇综述概述了活体生物成像中生物正交点击化学的最新发展。通常在体内系统及其体内进行的生物正交点击反应讨论了生物标记和成像的应用，特别是用于生物分子过程，治疗性细胞，肿瘤和细菌的可视化。还介绍了这个令人兴奋的领域的潜在未来方向。