Abstract This study is aimed to monitor the therapeutic response in breast cancer patients, by using the Raman spectra of liquid blood plasma of biopsy proved 19 breast cancer patients under pre and post treatment conditions and also by comparing them with the spectra of control group, consisting of 26 normal subjects (with no significant history of illness) in the same age group. The results indicate that there exists a statistical significance in the spectral signatures between the normal and breast cancer patient’s blood plasma as well as between pre treated and post treated plasma, which may be attributed to the changes in the vibrational characteristics of some of the key biomolecules viz tyrosine, phenylalanine, methionine, tryptophan, lipids/phospholipids, amides, DNA base pairs(adenine and guanine) etc. Further, it is found that the spectral signatures of post treated plasma (after completion of the entire course of treatment) are almost matching with that of normal subjects. The results of the study indicates the possibility of using Raman Spectroscopy for diagnosis as well as for monitoring of treatment response in breast cancer patients.

中文翻译：

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使用血浆拉曼光谱分析在治疗前后条件下监测乳腺癌患者

摘要 本研究旨在监测乳腺癌患者的治疗反应，通过使用活检证实的 19 名乳腺癌患者在治疗前后条件下的液体血浆的拉曼光谱，并将其与对照组的光谱进行比较，包括同一年龄组的 26 名正常受试者（无显着病史）。结果表明，正常和乳腺癌患者血浆之间以及预处理和处理后血浆之间的光谱特征存在统计学显着性，这可能归因于一些关键生物分子的振动特征的变化。即酪氨酸、苯丙氨酸、蛋氨酸、色氨酸、脂质/磷脂、酰胺、DNA碱基对（腺嘌呤和鸟嘌呤）等。 此外，发现处理后血浆（完成整个治疗过程后）的光谱特征与正常受试者的光谱特征几乎匹配。研究结果表明使用拉曼光谱进行诊断以及监测乳腺癌患者治疗反应的可能性。