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Conventional and non-conventional applications of β-galactosidases.
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ( IF 2.5 ) Pub Date : 2019-09-05 , DOI: 10.1016/j.bbapap.2019.140271 Carlos Vera 1 , Cecilia Guerrero 2 , Carla Aburto 2 , Andrés Cordova 3 , Andrés Illanes 2
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ( IF 2.5 ) Pub Date : 2019-09-05 , DOI: 10.1016/j.bbapap.2019.140271 Carlos Vera 1 , Cecilia Guerrero 2 , Carla Aburto 2 , Andrés Cordova 3 , Andrés Illanes 2
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β-Galactosidase is one of the most important industrial enzymes, that has been used for many decades in the dairy industry. The main application of β-galactosidase is related to the production of low-lactose and lactose-free milk and dairy products, which are now common consumer goods in supermarket shelves. This is a well-established market that is expected to keep on growing as these products become more accessible to mid-income people worldwide. However, a fresh air has come into the β-galactosidase business as non-conventional applications arose in recent decades based on its transgalactosylation activity. This capacity is certainly a major asset for a commodity enzyme that can be used now as a catalyst for the upgrading of readily available and cheap lactose into high added-value glycosides in processes of organic synthesis in tune with green chemistry principles within the framework of sustainability. This is a reflection of a paradigm shift, where enzymes are now being considered as apt catalysts for the synthesis of valuable organic compounds. This article reviews the main applications of β-galactosidase, going from its conventional use related to its hydrolytic activity to the ongoing non-conventional applications in the synthesis of high added-value oligosaccharides based on its transgalactosylation activity.
中文翻译：
β-半乳糖苷酶的常规和非常规应用。
β-半乳糖苷酶是最重要的工业酶之一，已在乳品工业中使用了数十年。β-半乳糖苷酶的主要用途涉及生产低乳糖和无乳糖的牛奶和乳制品，这些产品现在已成为超市货架上的常见消费品。这是一个成熟的市场，随着世界范围内中等收入人群对这些产品的使用越来越广泛，预计该市场将继续增长。然而，由于近几十年来基于其半乳糖苷化活性的非常规应用，新鲜空气进入了β-半乳糖苷酶行业。这种能力无疑是商品酶的主要资产，现在可以用作催化剂，在有机合成过程中根据绿色化学原理在可持续性框架内将易得的廉价乳糖升级为高附加值的糖苷。 。这反映了范式的转变，现在酶被认为是合成有价值的有机化合物的合适催化剂。本文回顾了β-半乳糖苷酶的主要应用，从其与水解活性有关的常规用途到基于其半乳糖基化活性合成高附加值寡糖的非常规应用。如今，酶被认为是合成有价值的有机化合物的合适催化剂。本文回顾了β-半乳糖苷酶的主要应用，从其与水解活性有关的常规用途到基于其半乳糖基化活性合成高附加值寡糖的非常规应用。如今，酶被认为是合成有价值的有机化合物的合适催化剂。本文回顾了β-半乳糖苷酶的主要应用，从其与水解活性有关的常规用途到基于其半乳糖基化活性合成高附加值寡糖的非常规应用。
更新日期：2019-09-05
中文翻译：
β-半乳糖苷酶的常规和非常规应用。
β-半乳糖苷酶是最重要的工业酶之一，已在乳品工业中使用了数十年。β-半乳糖苷酶的主要用途涉及生产低乳糖和无乳糖的牛奶和乳制品，这些产品现在已成为超市货架上的常见消费品。这是一个成熟的市场，随着世界范围内中等收入人群对这些产品的使用越来越广泛，预计该市场将继续增长。然而，由于近几十年来基于其半乳糖苷化活性的非常规应用，新鲜空气进入了β-半乳糖苷酶行业。这种能力无疑是商品酶的主要资产，现在可以用作催化剂，在有机合成过程中根据绿色化学原理在可持续性框架内将易得的廉价乳糖升级为高附加值的糖苷。 。这反映了范式的转变，现在酶被认为是合成有价值的有机化合物的合适催化剂。本文回顾了β-半乳糖苷酶的主要应用，从其与水解活性有关的常规用途到基于其半乳糖基化活性合成高附加值寡糖的非常规应用。如今，酶被认为是合成有价值的有机化合物的合适催化剂。本文回顾了β-半乳糖苷酶的主要应用，从其与水解活性有关的常规用途到基于其半乳糖基化活性合成高附加值寡糖的非常规应用。如今，酶被认为是合成有价值的有机化合物的合适催化剂。本文回顾了β-半乳糖苷酶的主要应用，从其与水解活性有关的常规用途到基于其半乳糖基化活性合成高附加值寡糖的非常规应用。
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