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Expression and role of CYP505A1 in pathogenicity of Fusarium oxysporum f. sp. lactucae.
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ( IF 2.5 ) Pub Date : 2019-09-03 , DOI: 10.1016/j.bbapap.2019.140268 Daniela Minerdi 1 , Sheila J Sadeghi 1 , Lara Pautasso 1 , Simone Morra 2 , Riccardo Aigotti 3 , Claudio Medana 3 , Giovanna Gilardi 4 , Maria Lodovica Gullino 4 , Gianfranco Gilardi 1
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ( IF 2.5 ) Pub Date : 2019-09-03 , DOI: 10.1016/j.bbapap.2019.140268 Daniela Minerdi 1 , Sheila J Sadeghi 1 , Lara Pautasso 1 , Simone Morra 2 , Riccardo Aigotti 3 , Claudio Medana 3 , Giovanna Gilardi 4 , Maria Lodovica Gullino 4 , Gianfranco Gilardi 1
Affiliation
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BACKGROUND
Cytochrome P450 enzymes (CYPs) are monooxygenases present in every domain of life. In fungi CYPs are involved in virulence. Fusarium wilt of lettuce, caused by F. oxysporum f. sp. lactucae, is the most serious disease of lettuce. F. oxysporum f.sp. lactucae MSA35 is an antagonistic fungus. Pathogenic formae specialis of F. oxysporum possess a CYP belonging to the new family CYP505. This enzyme hydroxylates saturated fatty acids that play a role in plant defence.
METHODS
Molecular tools were adopted to search for cyp505 gene in MSA35 genome. cyp505 gene expression analysis in pathogenic and antagonistic Fusarium was performed. The enzyme was expressed in its recombinant form and used for catalytic reactions with fatty acids, the products of which were characterized by mass spectrometry analysis.
RESULTS
A novel MSA35 self-sufficient CYP505 is differentially expressed in antagonistic and pathogenic F. oxysporum. Its expression is induced by the host plant lettuce in both pathogenesis and antagonism during the early phase of the interaction, while it is silenced during the late phase only in antagonistic Fusarium. Mass-spectrometry investigations proved that CYP505A1 mono-hydroxylates lauric, palmitic and stearic acids.
CONCLUSIONS
The ability of CYP505A1 to oxidize fatty acids present in the cortical cell membranes together with its differential expression in its Fusarium antagonistic form point out to the possibility that this enzyme is associated with Fusarium pathogenicity in lettuce.
GENERAL SIGNIFICANCE
The CYP505 clan is present in pathogenic fungal phyla, making CYP505A1 enzyme a putative candidate as a new target for the development of novel antifungal molecules.
中文翻译：
CYP505A1在尖孢镰刀菌致病性中的表达及作用。 sp。莴苣科。
背景技术细胞色素P450酶(CYP)是存在于生命各个领域的单加氧酶。在真菌中，CYP 参与毒力。生菜枯萎病，由 F. oxysporum f. 引起。 sp。生菜病害，是生菜最严重的病害。尖镰孢专化型乳酸菌 MSA35 是一种拮抗真菌。尖孢镰刀菌的致病性特形菌拥有属于新家族 CYP505 的 CYP。这种酶使饱和脂肪酸羟基化，在植物防御中发挥作用。方法采用分子工具在MSA35基因组中寻找cyp505基因。对致病性和拮抗性镰刀菌中的 cyp505 基因表达进行了分析。该酶以其重组形式表达并用于与脂肪酸的催化反应，其产物通过质谱分析进行表征。结果 新型 MSA35 自给自足的 CYP505 在拮抗性和致病性尖孢镰刀菌中差异表达。在相互作用的早期阶段，它的表达在发病机制和拮抗作用中均受到宿主植物生菜的诱导，而仅在拮抗性镰刀菌属中在晚期阶段被沉默。质谱研究证明 CYP505A1 可使月桂酸、棕榈酸和硬脂酸单羟基化。结论 CYP505A1 氧化皮质细胞膜中存在的脂肪酸的能力及其在镰刀菌拮抗形式中的差异表达表明该酶可能与生菜中镰刀菌的致病性有关。一般意义 CYP505 家族存在于致病真菌门中，使得 CYP505A1 酶成为开发新型抗真菌分子的新靶标的假定候选者。
更新日期：2019-10-25
中文翻译：
CYP505A1在尖孢镰刀菌致病性中的表达及作用。 sp。莴苣科。
背景技术细胞色素P450酶(CYP)是存在于生命各个领域的单加氧酶。在真菌中，CYP 参与毒力。生菜枯萎病，由 F. oxysporum f. 引起。 sp。生菜病害，是生菜最严重的病害。尖镰孢专化型乳酸菌 MSA35 是一种拮抗真菌。尖孢镰刀菌的致病性特形菌拥有属于新家族 CYP505 的 CYP。这种酶使饱和脂肪酸羟基化，在植物防御中发挥作用。方法采用分子工具在MSA35基因组中寻找cyp505基因。对致病性和拮抗性镰刀菌中的 cyp505 基因表达进行了分析。该酶以其重组形式表达并用于与脂肪酸的催化反应，其产物通过质谱分析进行表征。结果 新型 MSA35 自给自足的 CYP505 在拮抗性和致病性尖孢镰刀菌中差异表达。在相互作用的早期阶段，它的表达在发病机制和拮抗作用中均受到宿主植物生菜的诱导，而仅在拮抗性镰刀菌属中在晚期阶段被沉默。质谱研究证明 CYP505A1 可使月桂酸、棕榈酸和硬脂酸单羟基化。结论 CYP505A1 氧化皮质细胞膜中存在的脂肪酸的能力及其在镰刀菌拮抗形式中的差异表达表明该酶可能与生菜中镰刀菌的致病性有关。一般意义 CYP505 家族存在于致病真菌门中，使得 CYP505A1 酶成为开发新型抗真菌分子的新靶标的假定候选者。
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