Polycyclic Aromatic Hydrocarbons (PAHs) are complex compounds generated from industrial and anthropogenic activities, although natural phenomena are also responsible for their presence in the environment. The aim of this study was to evaluate the effect of phenanthrene on proximate composition and antioxidant activity in Piaractus brachypomus during a subacute exposure. Fish were exposed intraperitoneally to phenanthrene (0.1, 1.0, and 10 μg g-1) and a solvent control (0 μg g-1). Muscle tissue, liver and bile were collected at 0 h, 11 days and 21 days. In dorsal muscle, parameters such as dry matter, protein, crude fat, and pH did not show alterations with exposure to phenanthrene (p ≥ 0.05). At 11 days, a significant increase of hepatic lipid peroxidation was observed in fish exposed to 10 μg g-1. Additionally, a dose-dependent response was observed in the muscle, although no significant differences were observed in the activity of catalase and lipid peroxidation. Phenanthrene metabolites in bile were analyzed by fixed fluorescence at 260/380 nm (excitation/emission) wavelengths and levels indicated a dose-dependent response. Likewise, bioaccumulation of phenanthrene in bile was observed for 21 days, suggesting slow metabolism of this xenobiotic at 10 μg g-1. This study provides important information on the oxidative effects generated by phenanthrene exposure for 21 days in P. brachypomus, generating fundamental information on adverse effects induced by PAHs on the most important Colombian native fish species. These data contribute to the development of additional research in environments contaminated with PAHs and can be applied to monitor areas contaminated with oil spills.

中文翻译：

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暴露于菲的短螺旋体（Piaractus brachypomus）（双鱼座：Characidae）的生化反应和近期分析。

多环芳烃（PAHs）是由工业和人为活动产生的复杂化合物，尽管自然现象也是造成其在环境中存在的原因。这项研究的目的是评估在亚急性暴露过程中菲对近亲短鞭毛象的成分和抗氧化活性的影响。将鱼腹膜内暴露于菲（0.1、1.0和10μgg-1）和溶剂对照（0μgg-1）中。在0小时，11天和21天收集肌肉组织，肝和胆汁。在背肌中，诸如菲，蛋白质，粗脂肪和pH的参数没有显示出与菲接触的变化（p≥0.05）。在第11天，在暴露于10μgg-1的鱼中观察到肝脂质过氧化的显着增加。此外，尽管在过氧化氢酶和脂质过氧化的活性方面没有观察到显着差异，但在肌肉中观察到了剂量依赖性反应。通过固定的荧光在260/380 nm（激发/发射）波长下分析胆汁中的菲代谢物，其水平表明剂量依赖性反应。同样，在胆汁中观察到菲的生物富集21天，表明该异生素以10μgg-1的速度缓慢代谢。这项研究提供了重要的信息，即在21天的菲对虾中，菲暴露所产生的氧化作用，从而产生了有关PAHs对最重要的哥伦比亚本土鱼类造成的不利影响的基本信息。