Urinary glycosaminoglycans (GAGs) can reflect the health condition of a human being, and the GAGs composition can be directly related to various diseases. In order to effectively utilize such information, a detailed understanding of urinary GAGs in healthy individuals can provide insight into the levels and structures of human urinary GAGs. In this study, urinary GAGs were collected and purified from healthy males and females of adults and young adults. The total creatinine-normalized urinary GAG content, molecular weight distribution and disaccharide compositions were determined. Using capillary zone electrophoresis (CZE)-mass spectrometry (MS) and CZE-MS/MS relying on negative electron transfer dissociation, the major components of healthy human urinary GAGs were determined. The structures of 10 GAG oligosaccharides representing the majority of human urinary GAGs were determined.

中文翻译：

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健康人类受试者尿糖胺聚糖的结构分析。


尿糖胺聚糖（GAGs）可以反映人体的健康状况，而GAGs的组成与多种疾病有直接关系。为了有效利用这些信息，详细了解健康个体的尿液 GAG 可以深入了解人类尿液 GAG 的水平和结构。在这项研究中，从成人和年轻人的健康男性和女性中收集并纯化了尿 GAG。测定总肌酐归一化尿 GAG 含量、分子量分布和二糖组成。使用毛细管区带电泳 (CZE)-质谱 (MS) 和依靠负电子转移解离的 CZE-MS/MS，测定了健康人尿 GAG 的主要成分。确定了占人类尿 GAG 大部分的 10 种 GAG 寡糖的结构。