Spatially-resolved localization and chemical speciation of nickel and zinc in Noccaea tymphaea and Bornmuellera emarginata.,Metallomics

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Spatially-resolved localization and chemical speciation of nickel and zinc in Noccaea tymphaea and Bornmuellera emarginata.
Metallomics ( IF 2.9 ) Pub Date : 2019-12-11 , DOI: 10.1039/c9mt00106a
Antony van der Ent ₁ , Kathryn M Spiers , Dennis Brueckner , Guillaume Echevarria , Mark G M Aarts , Emmanuelle Montargès-Pelletier

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Hyperaccumulator plants present the ideal model system for studying the physiological regulation of the essential (and potentially toxic) transition elements nickel and zinc. This study used synchrotron X-ray Fluorescence Microscopy (XFM) elemental imaging and spatially resolved X-ray Absorption Spectroscopy (XAS) to elucidate elemental localization and chemical speciation of nickel and zinc in the hyperaccumulators Noccaea tymphaea and Bornmuellera emarginata (synonym Leptoplax emarginata). The results show that in the leaves of N. tymphaea nickel and zinc have contrasting localization, and whereas nickel is present in vacuoles of epidermal cells, zinc occurs mainly in the mesophyll cells. In the seeds Ni and Zn are similarly localized and strongly enriched in the cotyledons in N. tymphaea. Nickel is strongly enriched in the tip of the radicle of B. emarginata. Noccaea tymphaea has an Fe-rich provascular strand network in the cotyledons of the seed. The chemical speciation of Ni in the seeds of N. tymphaea is unequivocally associated with carboxylic acids, whereas Zn is present as the phytate complex. The spatially resolved spectroscopy did not reveal any spatial variation in chemical speciation of Ni and Zn within the N. tymphaea seed. The dissimilar ecophysiological behaviour of Ni and Zn in N. tymphaea and B. emarginata raises questions about the evolution of hyperaccumulation in these species.

中文翻译：

夜蛾夜蛾和Bornmuellera emarginata中镍和锌的空间分辨定位和化学形态。

超级蓄积植物为研究必需（和潜在有毒）过渡元素镍和锌的生理调节提供了理想的模型系统。这项研究使用同步加速器X射线荧光显微镜（XFM）元素成像和空间分辨X射线吸收光谱（XAS）阐明了超级蓄积型夜蛾夜蛾和Bornmuellera emarginata（同义词Leptoplax emarginata）中镍和锌的元素定位和化学形态。结果表明，在鼠伤寒叶中，镍和锌具有相反的定位，而表皮细胞的液泡中存在镍，而锌主要存在于叶肉细胞中。在种子中，Ni和Zn相似地定位于并在鼓膜猪笼草的子叶中大量富集。镍在B. emarginata胚根的顶端强烈富集。鼠夜蛾在种子的子叶中具有富铁的血管链网络。猪气N种子中Ni的化学形态与羧酸明确相关，而Zn以肌醇六磷酸配合物形式存在。空间分辨光谱学未显示出在睡莲种子内Ni和Zn的化学形态有任何空间变化。镍和锌在鼓膜猪笼草和B. emarginata中的不同生理生态行为提出了有关这些物种超积累进化的问题。锌以肌醇六磷酸配合物形式存在。空间分辨光谱学未显示出在睡莲种子内Ni和Zn的化学形态有任何空间变化。镍和锌在鼓膜猪笼草和线虫B. emarginata中的不同生理生态行为提出了有关这些物种超积累进化的问题。锌以肌醇六磷酸配合物的形式存在。空间分辨光谱学未显示出在睡莲种子内Ni和Zn的化学形态有任何空间变化。镍和锌在鼓膜猪笼草和B. emarginata中的不同生理生态行为提出了有关这些物种超积累进化的问题。

更新日期：2019-12-11

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