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Dual-emission fluorescent silicon nanoparticle-based nanothermometer for ratiometric detection of intracellular temperature in living cells.
Faraday Discussions ( IF 3.3 ) Pub Date : 2019-10-25 , DOI: 10.1039/c9fd00088g
Jinhua Wang 1 , Airui Jiang , Jingyang Wang , Bin Song , Yao He
Affiliation  

In this article, we present a kind of dual-emission fluorescent nanothermometer, which is made of europium (Eu3+)-doped silicon nanoparticles (Eu@SiNPs), allowing the detection of intracellular temperature in living cells with high accuracy. In particular, the presented SiNP-based thermometer features dual-emission fluorescence (blue (455 nm) and red (620 nm) emission), negligible toxicity (cell viability of treated cells remains above 90% during 24 h of treatment) and robust photostability in living cells (i.e., preserving >90% of fluorescence intensity after 45 min of continuous UV irradiation). More significantly, the fluorescence intensity of the Eu@SiNPs exhibits a linear ratiometric temperature response in a broad range from 25 to 70 °C. Taking advantage of these attractive merits, the Eu@SiNP-based nanothermometer is able to accurately (∼4.5% change per °C) determine dynamic changes in intracellular temperature in a quantitative and long-term (i.e., 30 min) manner.

中文翻译:

基于双发射荧光硅纳米粒子的纳米温度计,用于按比例检测活细胞中的细胞内温度。

在本文中,我们介绍了一种由fluorescent(Eu 3+)掺杂的硅纳米颗粒(Eu @ SiNPs)制成的双发射荧光纳米温度计,可以高精度检测活细胞的细胞内温度。特别是,本发明的基于SiNP的温度计具有双重发射荧光(蓝色(455 nm)和红色(620 nm)发射),毒性可忽略不计(在处理24小时内,处理过的细胞的细胞活力保持在90%以上)和强大的光稳定性。在活细胞中(,在连续UV照射45分钟后,荧光强度保持> 90%)。更重要的是,Eu @ SiNPs的荧光强度在25至70°C的宽范围内表现出线性比例温度响应。利用这些引人注目的优点,基于Eu @ SiNP的纳米温度计可以准确地(每°C改变约4.5%)以定量和长期(30分钟)的方式确定细胞内温度的动态变化。
更新日期:2019-10-25
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