Mesenchymal stem cells (MSCs) transplantation seems to be a promising new therapy for diabetic wound healing (DWH), and currently, arrays of MSCs from various sources ranging from umbilical, adipose to dental sources are available as a treatment modality for this disease. However, it now appears that only a fraction of transplanted cells actually assimilate and survive in host tissues suggesting that the major mechanism by which stem cells participate in tissue repair are most likely related to their secretome level. These include a wide range of growth factors, cytokines, and chemokines, which can be found from the conditioned medium (CM) used to culture the cells. Basic studies and preclinical work confirm that the therapeutic effect of CMs are comparable with the application of stem cells. This review describes in detail the wound healing process in diabetes and the cellular and biological factors that influence the process. Subsequently, through a comprehensive literature search of studies related to wound healing in diabetics, we aim to provide an overview of scientific merits of using MSCs-CM in the treatment of diabetic wound as well as the significant caveats, which restricts its potential use in clinical set-ups. To our best knowledge, this is one of the first review papers that collect the importance of stem cells as an alternative treatment to the DWH. We anticipate that the success of this treatment will have a significant clinical impact on diabetic wounds.

中文翻译：

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了解糖尿病伤口愈合的多方面机制以及干细胞条件培养基在愈合过程中的治疗应用。

间充质干细胞（MSCs）移植似乎是一种有前途的糖尿病伤口愈合（DWH）新疗法，目前，从脐带，脂肪到牙齿等多种来源的MSCs均可作为该疾病的治疗手段。但是，现在看来，只有一部分移植的细胞实际上在宿主组织中吸收并存活，这表明干细胞参与组织修复的主要机制很可能与它们的分泌组水平有关。这些包括多种生长因子，细胞因子和趋化因子，可以从用于培养细胞的条件培养基（CM）中找到。基础研究和临床前工作证实，CM的治疗效果与干细胞的应用相当。这篇综述详细描述了糖尿病的伤口愈合过程以及影响该过程的细胞和生物学因素。随后，通过对与糖尿病患者伤口愈合相关的研究进行全面的文献检索，我们旨在概述使用MSCs-CM治疗糖尿病伤口的科学优点以及一些重大警告，这限制了其在临床中的潜在应用设置。据我们所知，这是最早收集干细胞作为DWH替代治疗方法的重要性的综述论文之一。我们预计该治疗的成功将对糖尿病伤口产生重大的临床影响。通过对与糖尿病患者伤口愈合相关的研究进行全面的文献检索，我们旨在概述使用MSCs-CM来治疗糖尿病伤口的科学优点以及重大的警告，这限制了其在临床治疗中的潜在应用。 UPS。据我们所知，这是收集干细胞作为DWH替代疗法的重要性的第一批综述论文之一。我们预计该治疗的成功将对糖尿病伤口产生重大的临床影响。通过对与糖尿病患者伤口愈合相关的研究进行全面的文献检索，我们旨在概述使用MSCs-CM来治疗糖尿病伤口的科学优点以及重大的警告，这限制了其在临床治疗中的潜在应用。 UPS。据我们所知，这是最早收集干细胞作为DWH替代治疗方法的重要性的综述论文之一。我们预计该治疗的成功将对糖尿病伤口产生重大的临床影响。这是收集干细胞作为DWH替代疗法的重要性的第一批综述论文之一。我们预计该治疗的成功将对糖尿病伤口产生重大的临床影响。这是收集干细胞作为DWH替代疗法的重要性的第一批综述论文之一。我们预计该治疗的成功将对糖尿病伤口产生重大的临床影响。