Abstract A highly selective and effective method for the purification and preconcentration of norfloxacin (NFX) in seawater samples was developed based on molecularly imprinted solid-phase extraction (MISPE). The molecularly imprinted polymer was synthesized by precipitation polymerization. Methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) were used as the functional monomer and crosslinker, respectively. The resulting molecularly imprinted polymer (MIP) showed high adsorption for NFX and was selective for its solid-phase extraction. An offline MISPE method followed by high performance liquid chromatography with diode array detection was established for the determination of NFX in seawater. The recoveries of spiked seawater samples using the MISPE columns were satisfactorily higher than 77.6%. The relative standard deviation was less than 5.60%, and the limit of detection was 0.027 μg L−1. Four seawater samples obtained from the Bohai Sea were analyzed, and NFX was found only at one location at a concentration of 0.280 μg L−1.

中文翻译：

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使用分子印迹聚合物进行固相萃取，选择性纯化和预浓缩海水中的诺氟沙星

摘要 建立了一种基于分子印迹固相萃取（MISPE）的高选择性、高效的海水样品中诺氟沙星（NFX）的纯化和富集方法。分子印迹聚合物是通过沉淀聚合合成的。甲基丙烯酸（MAA）和乙二醇二甲基丙烯酸酯（EGDMA）分别用作功能单体和交联剂。所得分子印迹聚合物 (MIP) 对 NFX 显示出高吸附性，并且对其固相萃取具有选择性。建立了离线 MISPE 后接高效液相色谱与二极管阵列检测的方法，用于测定海水中的 NFX。使用 MISPE 色谱柱对加标海水样品的回收率令人满意地高于 77.6%。相对标准偏差小于5.60%，检测限为0.027 μg L-1。分析了从渤海获得的四个海水样品，仅在一处发现 NFX，浓度为 0.280 μg L-1。