Abstract Yellow mombin residue was investigated as a substrate for the production of xylanase by Penicillium roqueforti ATCC 10110 in solid-state fermentation. The parameters incubation temperature, initial moisture, and fermentation time were optimized using the Box–Behnken design, with a maximum yield of 14.03 IU g−1 at 25.55 °C, 60.55% moisture and 141.80 h, respectively. The xylanases produced showed higher activity and stability in pH in the range of 3–5 and temperature ≤ 60 °C. The respective Km and VMax values found were 1.96 mg mL−1 and 16.23 μmol min−1 mL−1. The addition of the Mn2+, Al3+, Zn2+, Na+, Ca2+ and Trolox and Triton X-100 compounds increased xylanase activity, whereas Mg2+, Pb2+, Cu2+ and SDS species behaved as inhibitors. Freezing at −20 or 4 °C maintained more than 94% of the enzymatic activity after 120 days of storage. Solid-state fermentation in yellow mombin residue, without any additives, using P. roqueforti ATCC 10110 allowed the acquisition of xylanases with characteristics similar to those obtained from processes using synthetic substrates, indicating a promising alternative for the xylanase obtaining.

中文翻译：

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罗克福青霉 ATCC 10110 固相发酵条件优化及木聚糖酶特性研究

摘要 研究了黄木宾残渣作为罗克福青霉 ATCC 10110 固态发酵生产木聚糖酶的底物。使用 Box-Behnken 设计优化了温育温度、初始水分和发酵时间参数，在 25.55°C、60.55% 水分和 141.80 小时的最大产量分别为 14.03 IU g-1。产生的木聚糖酶在 3-5 范围内的 pH 值和≤ 60 °C 的温度下显示出更高的活性和稳定性。发现的相应 Km 和 VMax 值为 1.96 mg mL-1 和 16.23 μmol min-1 mL-1。添加 Mn2+、Al3+、Zn2+、Na+、Ca2+ 和 Trolox 和 Triton X-100 化合物可提高木聚糖酶活性，而 Mg2+、Pb2+、Cu2+ 和 SDS 物质则表现为抑制剂。在 -20 或 4 °C 下冷冻保存 120 天后，酶活性保持在 94% 以上。