The analysis of monoclonal antibodies (mAbs) by a middle-down mass spectrometry (MS) approach is a growing field that attracts the attention of many researchers and biopharmaceutical companies. Usually, liquid fractionation techniques are used to separate mAbs polypeptides chains before MS analysis. Gas-phase fractionation techniques such as high-field asymmetric waveform ion mobility spectrometry (FAIMS) can replace liquid-based separations and reduce both analysis time and cost. Here, we present a rapid FAIMS tandem MS method capable of characterizing the polypeptide sequence of mAbs light and heavy chains in an unprecedented, easy, and fast fashion. This new method uses commercially available instruments and takes ~24 min, which is 40-60% faster than regular liquid chromatography-MS/MS analysis, to acquire fragmentation data using different dissociation methods.

通过中下质谱（MS）方法分析单克隆抗体（mAb）是一个正在发展的领域，吸引了许多研究人员和生物制药公司的关注。通常，在MS分析之前，使用液体分级分离技术来分离mAb多肽链。气相分馏技术（例如高场非对称波形离子迁移谱法（FAIMS））可以代替基于液体的分离，并减少分析时间和成本。在这里，我们提出了一种快速的FAIMS串联MS方法，该方法能够以空前，简便和快速的方式表征mAbs轻链和重链的多肽序列。这种新方法使用市售仪器，耗时约24分钟，比常规液相色谱-MS / MS分析快40-60％，