We aimed to define whether embryo collection carried out after pseudopregnancy was of similar outcome and quality as after artificial abortion. To induce pseudopregnancy, 30 gilts or sows were given 20 mg intramuscular estradiol dipropionate (EDP) 10-11 days after the onset of estrus. Ten additional pigs were inseminated artificially at natural estrus as a control group. Prostaglandin F2α (PGF2α ) was administered twice with a 24 hr interval beginning 15, 20, or 25 days after EDP-treatment (n = 10 per group) or between 23 and 39 days after artificial insemination in control pigs. Following this, all pigs were given 1,000 IU equine chorionic gonadotropin and 500 IU human chorionic gonadotropin (hCG) and then inseminated. Embryos were recovered 6 or 7 days after hCG treatment and outcome was recorded. There was no significant difference in the number of normal embryos collected from the pigs with PGF2α initiated at different time points or from the control group. Embryonic developmental stages 7 days after hCG treatment also did not differ among groups. These results indicate that the use of EDP to induce pseudopregnancy, followed by PGF2α administration to synchronize estrus for subsequent embryo harvest, is a suitable alternative to the artificial abortion method.

中文翻译：

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丙二醇雌二醇诱导的假性妊娠后猪的胚胎收集。

我们旨在确定假怀孕后进行的胚胎收集是否与人工流产后具有相似的结局和质量。为了诱发假孕，在发情开始后10-11天给30头小母猪或母猪服用20 mg肌内雌二醇二丙酸酯（EDP）。作为自然对照组，在自然发情时对另外十头猪进行人工授精。前列腺素F2α（PGF2α）在EDP治疗后第15、20或25天（每组n = 10）或人工授精后23到39天之间以24小时间隔两次给药（每组n = 10）。此后，给所有猪提供1,000 IU马绒毛膜促性腺激素和500 IU人绒毛膜促性腺激素（hCG），然后进行授精。hCG治疗后6或7天恢复了胚胎，并记录了结局。从在不同时间点启动的PGF2α的猪或对照组收集的正常胚胎的数量没有显着差异。hCG治疗后7天的胚胎发育阶段在各组之间也没有差异。这些结果表明，使用EDP诱导假性妊娠，然后施用PGF2α同步发情以进行随后的胚胎收获，是人工流产方法的合适替代方法。