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Effective oral delivery of gp100 plasmid vaccine against metastatic melanoma through multi-faceted blending-by-blending nanogels.
Nanomedicine: Nanotechnology, Biology and Medicine ( IF 4.2 ) Pub Date : 2019-10-23 , DOI: 10.1016/j.nano.2019.102114 Yurun Shen 1 , Liyan Qiu 1
Nanomedicine: Nanotechnology, Biology and Medicine ( IF 4.2 ) Pub Date : 2019-10-23 , DOI: 10.1016/j.nano.2019.102114 Yurun Shen 1 , Liyan Qiu 1
Affiliation
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Plasmid DNA gp100 is able to act as an available vaccine against metastatic melanoma, but its administration is usually limited to parenteral route. Since oral delivery of plasmid DNA is intervened by various physical obstacles, here we constructed a nanogel (Alg-Tat-gp100) with multi-faceted functions through blending-by-blending method. Due to the cooperation of alginate and Tat peptide, Alg-Tat-gp100 demonstrated the significant improvement of stability in the stomach, mucus penetration ability in intestine, and transport across mucus layer and MDCK cells. Moreover, the bone marrow-derived cells were activated with an enhanced co-stimulatory molecule expression. Following immunization using Alg-Tat-gp100 nanogels in C57BL/6 mice, the secretion of IFN-γ and the activation of cytotoxic T cells were significantly improved. Benefiting from those cases, the B16F10 tumor inhibition rate achieved 42.5% by this oral DNA vaccine, suggesting that this multi-faceted nanogel prepared by simple blending-by-blending method may provide a new strategy for oral DNA vaccine delivery.
中文翻译：
通过多面融合纳米凝胶有效地口服递送针对转移性黑色素瘤的gp100质粒疫苗。
质粒DNA gp100可以作为抗转移性黑色素瘤的可用疫苗，但其给药通常限于肠胃外途径。由于质粒DNA的口服递送受到各种物理障碍的干扰，因此在这里我们通过掺混法构建了具有多方面功能的纳米凝胶（Alg-Tat-gp100）。由于藻酸盐和Tat肽的协同作用，Alg-Tat-gp100证明了胃的稳定性，粘液在肠中的渗透能力以及跨粘液层和MDCK细胞运输的显着改善。此外，以增强的共刺激分子表达激活了骨髓来源的细胞。在C57BL / 6小鼠中使用Alg-Tat-gp100纳米凝胶免疫后，IFN-γ的分泌和细胞毒性T细胞的活化得到了显着改善。
更新日期：2019-10-23
中文翻译：
通过多面融合纳米凝胶有效地口服递送针对转移性黑色素瘤的gp100质粒疫苗。
质粒DNA gp100可以作为抗转移性黑色素瘤的可用疫苗，但其给药通常限于肠胃外途径。由于质粒DNA的口服递送受到各种物理障碍的干扰，因此在这里我们通过掺混法构建了具有多方面功能的纳米凝胶（Alg-Tat-gp100）。由于藻酸盐和Tat肽的协同作用，Alg-Tat-gp100证明了胃的稳定性，粘液在肠中的渗透能力以及跨粘液层和MDCK细胞运输的显着改善。此外，以增强的共刺激分子表达激活了骨髓来源的细胞。在C57BL / 6小鼠中使用Alg-Tat-gp100纳米凝胶免疫后，IFN-γ的分泌和细胞毒性T细胞的活化得到了显着改善。
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