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Survival of silk worm, Bombyx mori in azaserine induced oxidative stress.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2019-10-23 , DOI: 10.1016/j.cbpc.2019.108637
Venkatesh Mandyam D 1 , Subramanyam Muthangi 1
Affiliation  

Cells under stress generate reactive oxygen species (ROS) in excess, which causes mitochondrial dysfunction and stimulates the apoptotic cascade. However, mild stress or pre-conditioning lead to the evasion of apoptosis by activating mitogenic signaling, including the signaling of inhibitors of apoptosis proteins (IAPs), or by inactivating certain apoptotic molecules. The silkworm (Bombyx mori) is an important economic insect which serves as a model organism in biological research. Bombyx mori apoptotic protease inducing factor (BmApaf1), a death-related ced-3/Nedd2-like protein (BmDredd), and BmSurvivin-2 (BmSvv2) are known to play significant roles in metamorphosis. Azaserine is an analogue of glutamine and irreversibly inhibits glutamine-utilizing enzymes and cysteine-glutamate transporter genes EAAT2. In the present study, we experimentally demonstrated stress induced by azaserine along with the capacity of antioxidants to modulate apoptotic/anti-apoptotic gene expression in determining the fate of the larvae. We observed higher larval survival with higher azaserine dosages and attributed this to the quantum of ROS generated and AOEs response, which favoured the BmSvv2 expression. Meanwhile higher levels of ROS with concomitant changes in AOEs were found to be responsible for BmApaf1 and BmDredd expression, which reflected a higher mortality rate.

中文翻译:

蚕,天蚕中的家蚕的生存诱导了氧化应激。

处于压力下的细胞会产生过量的活性氧(ROS),这会导致线粒体功能障碍并刺激凋亡级联反应。但是,轻度的压力或预处理会通过激活促有丝分裂信号传导(包括凋亡蛋白(IAPs)抑制剂的信号传导或使某些凋亡分子失活而导致细胞凋亡。蚕(Bombyx mori)是一种重要的经济昆虫,在生物学研究中可作为典范生物。已知家蚕凋亡蛋白酶诱导因子(BmApaf1),与死亡相关的ced-3 / Nedd2样蛋白(BmDredd)和BmSurvivin-2(BmSvv2)在变态中起重要作用。Azaserine是谷氨酰胺的类似物,不可逆地抑制利用谷氨酰胺的酶和半胱氨酸-谷氨酸转运蛋白基因EAAT2。在目前的研究中,我们通过实验证明了天青素诱导的应激以及抗氧化剂调节幼虫命运的调节凋亡/抗凋亡基因表达的能力。我们观察到较高的叠氮碱剂量具有较高的幼虫存活率,并将其归因于产生的ROS量和AOEs响应,这有利于BmSvv2表达。同时,发现较高水平的ROS和AOE伴随变化是BmApaf1和BmDredd表达的原因,这反映了较高的死亡率。偏爱BmSvv2表达。同时,发现较高水平的ROS和AOE伴随变化是BmApaf1和BmDredd表达的原因,这反映了较高的死亡率。偏爱BmSvv2表达。同时,发现较高水平的ROS和AOE伴随变化是BmApaf1和BmDredd表达的原因,这反映了较高的死亡率。
更新日期:2019-10-23
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