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Candida glabrata peroxiredoxins, Tsa1 and Tsa2, and sulfiredoxin, Srx1, protect against oxidative damage and are necessary for virulence.
Fungal Genetics and Biology ( IF 2.4 ) Pub Date : 2019-10-22 , DOI: 10.1016/j.fgb.2019.103287 Guadalupe Gutiérrez-Escobedo 1 , Oscar Hernández-Carreón 1 , Brenda Morales-Rojano 1 , Brenda Revuelta-Rodríguez 1 , Norma Vázquez-Franco 1 , Irene Castaño 1 , Alejandro De Las Peñas 1
Fungal Genetics and Biology ( IF 2.4 ) Pub Date : 2019-10-22 , DOI: 10.1016/j.fgb.2019.103287 Guadalupe Gutiérrez-Escobedo 1 , Oscar Hernández-Carreón 1 , Brenda Morales-Rojano 1 , Brenda Revuelta-Rodríguez 1 , Norma Vázquez-Franco 1 , Irene Castaño 1 , Alejandro De Las Peñas 1
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Candida glabrata is an opportunistic fungal pathogen that can cause life-threatening infections in immunocompromised patients. To ensure a successful infection, C. glabrata has evolved a variety of strategies to avoid killing within the host. One of these strategies is the resistance to oxidative stress. Here we show that the sulfiredoxin Srx1 and the peroxiredoxins, Tsa1 and Tsa2, are implicated in the oxidative stress response (OSR) and required for virulence. We analyzed null mutations in SRX1, TSA1 and TSA2 and showed that TSA2 and SRX1 are required to respond to oxidative stress. While TSA1 expression is constitutive, SRX1 and TSA2 are induced in the presence of H2O2 in a process dependent on H2O2 concentration and on both transcription factors Yap1 and Skn7. Msn2 and Msn4 are not necessary for the regulation of SRX1, TSA1 and TSA2. Interestingly, TSA1 and TSA2, which are localized in the cytoplasm, are induced in the presence of neutrophils and required for survival in these phagocytic cells.
中文翻译：
光滑念珠菌过氧化物酶Tsa1和Tsa2，以及硫脲毒素Srx1可以防止氧化损伤，并且对于毒力是必不可少的。
光滑念珠菌是一种机会性真菌病原体，可在免疫受损的患者中引起威胁生命的感染。为了确保成功感染，光滑念珠菌已开发出多种策略来避免在宿主内杀死。这些策略之一是抗氧化应激。在这里，我们显示了硫氧还蛋白Srx1和过氧化物氧还蛋白Tsa1和Tsa2与氧化应激反应（OSR）有关，并且是毒性所必需的。我们分析了SRX1，TSA1和TSA2中的无效突变，并表明TSA2和SRX1需要对氧化应激作出反应。虽然TSA1表达是组成型的，但在H2O2存在的情况下，SRX1和TSA2的诱导过程取决于H2O2的浓度以及转录因子Yap1和Skn7。Msn2和Msn4对于SRX1，TSA1和TSA2的调节不是必需的。
更新日期：2019-10-23
中文翻译：
光滑念珠菌过氧化物酶Tsa1和Tsa2，以及硫脲毒素Srx1可以防止氧化损伤，并且对于毒力是必不可少的。
光滑念珠菌是一种机会性真菌病原体，可在免疫受损的患者中引起威胁生命的感染。为了确保成功感染，光滑念珠菌已开发出多种策略来避免在宿主内杀死。这些策略之一是抗氧化应激。在这里，我们显示了硫氧还蛋白Srx1和过氧化物氧还蛋白Tsa1和Tsa2与氧化应激反应（OSR）有关，并且是毒性所必需的。我们分析了SRX1，TSA1和TSA2中的无效突变，并表明TSA2和SRX1需要对氧化应激作出反应。虽然TSA1表达是组成型的，但在H2O2存在的情况下，SRX1和TSA2的诱导过程取决于H2O2的浓度以及转录因子Yap1和Skn7。Msn2和Msn4对于SRX1，TSA1和TSA2的调节不是必需的。
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