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A Jasmonate-Activated MYC2-Dof2.1-MYC2 Transcriptional Loop Promotes Leaf Senescence in Arabidopsis.
The Plant Cell ( IF 10.0 ) Pub Date : 2019-10-22 , DOI: 10.1105/tpc.19.00297 Mengna Zhuo 1 , Yasuhito Sakuraba 1 , Shuichi Yanagisawa 2
The Plant Cell ( IF 10.0 ) Pub Date : 2019-10-22 , DOI: 10.1105/tpc.19.00297 Mengna Zhuo 1 , Yasuhito Sakuraba 1 , Shuichi Yanagisawa 2
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DNA binding-with-one-finger (Dof) proteins are plant-specific transcription factors closely associated with a variety of physiological processes. Here, we show that the Dof protein family in Arabidopsis (Arabidopsis thaliana) functions in leaf senescence. Disruption of Dof2 1, a jasmonate (JA)-inducible gene, led to a marked reduction in promotion of leaf senescence and inhibition of root development as well as dark-induced and age-dependent leaf senescence, while overexpression of Dof2 1 promoted these processes. Additionally, the dof2 1 knockout mutant showed almost no change in the transcriptome in the absence of JA; in the presence of JA, expression of many senescence-associated genes, including MYC2, which encodes a central regulator of JA responses, was induced to a lesser extent in the dof2 1 mutant than in the wild type. Furthermore, direct activation of the MYC2 promoter by Dof2.1, along with the results of epistasis analysis, indicated that Dof2.1 enhances leaf senescence mainly by promoting MYC2 expression. Interestingly, MYC2 was also identified as a transcriptional activator responsible for JA-inducible expression of Dof2 1 Based on these results, we propose that Dof2.1 acts as an enhancer of JA-induced leaf senescence through the MYC2-Dof2.1-MYC2 feedforward transcriptional loop.
中文翻译：
茉莉酸激活的MYC2-Dof2.1-MYC2转录环促进拟南芥叶片衰老。
DNA与一个手指结合（Dof）蛋白是与多种生理过程密切相关的植物特异性转录因子。在这里，我们显示了拟南芥（Arabidopsis thaliana）中的Dof蛋白家族在叶片衰老中起作用。茉莉酸（JA）诱导基因Dof2 1的破坏导致叶片衰老的促进和根系发育的抑制显着降低，以及黑暗诱导的和年龄依赖性的叶片衰老，而Dof2 1的过表达则促进了这些过程。 。另外，在没有JA的情况下，dof2 1敲除突变体在转录组中几乎没有变化。在存在JA的情况下，与野生型相比，在dof2 1突变体中诱导了许多衰老相关基因（包括MYC2的表达）的编码程度较低，其中MYC2编码JA响应的中央调节剂。此外，Dof2.1对MYC2启动子的直接激活以及上位性分析的结果表明，Dof2.1主要通过促进MYC2表达来增强叶片衰老。有趣的是，MYC2还被确定为负责JA诱导的Dof2 1表达的转录激活因子。基于这些结果，我们建议Dof2.1通过MYC2-Dof2.1-MYC2前馈作为JA诱导的叶片衰老的增强剂。转录环。
更新日期：2020-01-10
中文翻译：
茉莉酸激活的MYC2-Dof2.1-MYC2转录环促进拟南芥叶片衰老。
DNA与一个手指结合（Dof）蛋白是与多种生理过程密切相关的植物特异性转录因子。在这里，我们显示了拟南芥（Arabidopsis thaliana）中的Dof蛋白家族在叶片衰老中起作用。茉莉酸（JA）诱导基因Dof2 1的破坏导致叶片衰老的促进和根系发育的抑制显着降低，以及黑暗诱导的和年龄依赖性的叶片衰老，而Dof2 1的过表达则促进了这些过程。 。另外，在没有JA的情况下，dof2 1敲除突变体在转录组中几乎没有变化。在存在JA的情况下，与野生型相比，在dof2 1突变体中诱导了许多衰老相关基因（包括MYC2的表达）的编码程度较低，其中MYC2编码JA响应的中央调节剂。此外，Dof2.1对MYC2启动子的直接激活以及上位性分析的结果表明，Dof2.1主要通过促进MYC2表达来增强叶片衰老。有趣的是，MYC2还被确定为负责JA诱导的Dof2 1表达的转录激活因子。基于这些结果，我们建议Dof2.1通过MYC2-Dof2.1-MYC2前馈作为JA诱导的叶片衰老的增强剂。转录环。
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