We previously studied the phenomena of the mesenchymal cell-dependent mode of cartilage growth in quail and catfish. Thus, we selected the two cartilage models in which mesenchymal cells participate in their growth. In such models, cartilage degradation occurred to facilitate cellular invasion. The studies do not explain the nature of the cartilage degrading cells. The current study aims to explore the nature of the cartilage-degrading cells using transmission electron microscopy (TEM) and immunohistochemistry. Samples of cartilage have been isolated from the air-breathing organ of catfish and the cartilage of the prospective occipital bone of quail embryos. Samples have been processed for TEM and immunohistochemistry. We found that two different cell types are involved in cartilage degradation; the macrophage in the cartilage of catfish and mesenchymal cells in the cartilage of the quail. Areas of cellular invasion in both catfish cartilage and quail embryo cartilage had an immunological affinity for MMP-9. In catfish, cartilage-degrading cells had identical morphological features of macrophages, whereas in quail embryos, cartilage-degrading cells were mesenchymal-like cells which had cell processes rich in vesicles and expressed CD117. Further study should consider the role of macrophage and mesenchymal cells during cartilage degradation. This could be valuable to be applied to remove the defective cartilage matrix formed in osteoarthritic patients to improve cartilage repair strategies.

中文翻译：

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细胞入侵和基质降解，鲶鱼 (Clarias gariepinus) 和日本鹌鹑胚胎 (Coturnix coturnix japonica) 软骨中不同类型的基质降解细胞

我们之前研究了鹌鹑和鲶鱼软骨生长的间充质细胞依赖性模式的现象。因此，我们选择了间充质细胞参与其生长的两种软骨模型。在这样的模型中，发生软骨退化以促进细胞入侵。这些研究没有解释软骨降解细胞的性质。目前的研究旨在使用透射电子显微镜 (TEM) 和免疫组织化学来探索软骨降解细胞的性质。软骨样本已从鲶鱼的呼吸器官和鹌鹑胚胎的枕骨软骨中分离出来。样品已经过 TEM 和免疫组织化学处理。我们发现两种不同的细胞类型与软骨退化有关。鲶鱼软骨中的巨噬细胞和鹌鹑软骨中的间充质细胞。鲶鱼软骨和鹌鹑胚胎软骨中的细胞侵袭区域对 MMP-9 具有免疫亲和性。在鲶鱼中，软骨降解细胞具有与巨噬细胞相同的形态特征，而在鹌鹑胚胎中，软骨降解细胞是间充质样细胞，其细胞突起富含囊泡并表达 CD117。进一步的研究应考虑巨噬细胞和间充质细胞在软骨降解过程中的作用。这对于去除骨关节炎患者中形成的有缺陷的软骨基质以改善软骨修复策略可能很有价值。鲶鱼软骨和鹌鹑胚胎软骨中的细胞侵袭区域对 MMP-9 具有免疫亲和性。在鲶鱼中，软骨降解细胞具有与巨噬细胞相同的形态特征，而在鹌鹑胚胎中，软骨降解细胞是间充质样细胞，其细胞突起富含囊泡并表达 CD117。进一步的研究应考虑巨噬细胞和间充质细胞在软骨降解过程中的作用。这对于去除骨关节炎患者中形成的有缺陷的软骨基质以改善软骨修复策略可能很有价值。鲶鱼软骨和鹌鹑胚胎软骨中的细胞侵袭区域对 MMP-9 具有免疫亲和性。在鲶鱼中，软骨降解细胞具有与巨噬细胞相同的形态特征，而在鹌鹑胚胎中，软骨降解细胞是间充质样细胞，其细胞突起富含囊泡并表达 CD117。进一步的研究应考虑巨噬细胞和间充质细胞在软骨降解过程中的作用。这对于去除骨关节炎患者中形成的有缺陷的软骨基质以改善软骨修复策略可能很有价值。进一步的研究应考虑巨噬细胞和间充质细胞在软骨降解过程中的作用。这对于去除骨关节炎患者中形成的有缺陷的软骨基质以改善软骨修复策略可能很有价值。进一步的研究应考虑巨噬细胞和间充质细胞在软骨降解过程中的作用。这对于去除骨关节炎患者中形成的有缺陷的软骨基质以改善软骨修复策略可能很有价值。