Expression of glut2 in response to glucose load, insulin and glucagon in grass carp (Ctenophcuyngodon idellus).,Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology

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Expression of glut2 in response to glucose load, insulin and glucagon in grass carp (Ctenophcuyngodon idellus).
Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology ( IF 1.9 ) Pub Date : 2019-09-10 , DOI: 10.1016/j.cbpb.2019.110351
Wenli Zhao ₁ , Chaobin Qin ₁ , Guokun Yang ₁ , Xiao Yan ₁ , Xiaolin Meng ₁ , Liping Yang ₁ , Ronghua Lu ₁ , Dapeng Deng ₁ , Mingming Niu ₁ , Guoxing Nie ₁

Affiliation

Generally, fish are thought to have a limited ability to utilize carbohydrate. Postprandial blood glucose is cleared sluggishly in fish, resulting in prolonged hyperglycemia. Facilitative glucose transporters (GLUTs) play an important role in glucose utilization. In the present study, the expression levels of glut2 in different tissues were detected in grass carp. Furthermore, the effects of oral glucose administration on glut2 mRNA expression in the liver, intestine and kidney were investigated, and we also evaluated the response of glut2 mRNA to insulin and glucagon in the primary hepatocytes of grass carp. The expression level of glut2 mRNA was highest in the liver, followed by the intestine and kidney, but lower in other tissues. The result of glucose tolerance test (GTT) showed that serum glucose reached the highest level at 3 h after GTT and recovered to the basic level at 6 h. The glut2 mRNA in the intestine was up-regulated at 1 h after GTT. However, the glut2 mRNA expression in the liver of grass carp was unchanged after GTT for 1, 3, 6 h, and even decreased at 12 h after GTT. In addition, the expression of glut2 mRNA in the primary hepatocytes was enhanced by insulin and glucagon at 3 h post treatment. These results suggested that glut2 expression in the liver of grass carp was sensitive to insulin and glucagon, but not blood glucose. The up-regulation of glut2 by these hormones might be involved in the bi-directional transportation of glucose in the liver.

中文翻译：

草鱼（Ctenophcuyngodon idellus）对葡萄糖负荷，胰岛素和胰高血糖素的响应而表达的glut2。

通常，人们认为鱼类利用碳水化合物的能力有限。鱼类餐后血糖缓慢清除，导致长时间的高血糖症。促进性葡萄糖转运蛋白（GLUT）在葡萄糖利用中起重要作用。在本研究中，检测了草鱼不同组织中glut2的表达水平。此外，研究了口服葡萄糖对肝脏，肠道和肾脏中glut2 mRNA表达的影响，我们还评估了草鱼原代肝细胞中glut2 mRNA对胰岛素和胰高血糖素的反应。glut2 mRNA的表达水平在肝脏中最高，其次是肠和肾，但在其他组织中较低。葡萄糖耐量试验（GTT）的结果表明，GTT后3小时血清葡萄糖达到最高水平，并在6小时后恢复到碱性水平。GTT后1小时，肠道中的glut2 mRNA上调。然而，GTT后1、3、6 h草鱼肝脏中的glut2 mRNA表达没有变化，甚至在GTT后12 h下降。另外，在治疗后3小时，胰岛素和胰高血糖素增强了原代肝细胞中的glut2 mRNA的表达。这些结果表明草鱼肝脏中的glut2表达对胰岛素和胰高血糖素敏感，但对血糖不敏感。这些激素对glut2的上调可能与肝脏中葡萄糖的双向运输有关。GTT后1、3、6 h草鱼肝脏中的glut2 mRNA表达没有变化，而在GTT后12 h甚至下降。另外，在治疗后3小时，胰岛素和胰高血糖素增强了原代肝细胞中的glut2 mRNA的表达。这些结果表明草鱼肝脏中的glut2表达对胰岛素和胰高血糖素敏感，但对血糖不敏感。这些激素对glut2的上调可能与肝脏中葡萄糖的双向运输有关。GTT后1、3、6 h草鱼肝脏中的glut2 mRNA表达没有变化，而在GTT后12 h甚至下降。另外，在治疗后3小时，胰岛素和胰高血糖素增强了原代肝细胞中的glut2 mRNA的表达。这些结果表明草鱼肝脏中的glut2表达对胰岛素和胰高血糖素敏感，但对血糖不敏感。这些激素对glut2的上调可能与肝脏中葡萄糖的双向运输有关。这些结果表明草鱼肝脏中的glut2表达对胰岛素和胰高血糖素敏感，但对血糖不敏感。这些激素对glut2的上调可能与肝脏中葡萄糖的双向运输有关。这些结果表明草鱼肝脏中的glut2表达对胰岛素和胰高血糖素敏感，但对血糖不敏感。这些激素对glut2的上调可能与肝脏中葡萄糖的双向运输有关。

更新日期：2019-09-10

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