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Identification of duplicated suppressor of cytokine signaling 3 (SOCS3) genes in blunt snout bream (Megalobrama amblycephala).
Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology ( IF 1.9 ) Pub Date : 2019-09-14 , DOI: 10.1016/j.cbpb.2019.110348 Shan-Shan Zhao 1 , Xin-Yu Zhao 1 , Cheng-Bin Wu 1 , Guo-Dong Zheng 1 , Shu-Ming Zou 1
Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology ( IF 1.9 ) Pub Date : 2019-09-14 , DOI: 10.1016/j.cbpb.2019.110348 Shan-Shan Zhao 1 , Xin-Yu Zhao 1 , Cheng-Bin Wu 1 , Guo-Dong Zheng 1 , Shu-Ming Zou 1
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The suppressor of cytokine signaling 3 (SOCS3) negatively regulates the responses of various immune cytokines. In this study, we identified socs3s genes of blunt snout bream. 209- and 216-aa long peptides are encoded by socs3a and socs3b genes, respectively. The socs3s mRNAs are expressed consistently during the entire process of embryonic development. Whole-mount in situ hybridization detected socs3a in the eyes and posterior somites at 12 h post fertilization (hpf), transcribed at the otic vesicle at 24 hpf, and transcribed at the eyes, brain, and otic vesicle at 36 hpf; while the socs3b mRNA was transcribed at the notochord at 12 hpf, expressed in the brain, eyes, and tailbud at 24 hpf, and detected in the brain at 36 hpf. The expression of socs3a is slightly different from that of socs3b in tissues of juvenile and adult blunt snout bream. After recombinant human growth hormone (hGH) treatment, the transcript levels of socs3s of blunt snout bream were increased in gills, spleen, kidney, and gonads. After Aerononas hydrophila infection, the mRNA levels of socs3s of blunt snout bream were significantly increased in the liver, spleen, intestine, and kidney tissues. Blunt snout bream were susceptible to various pathogenic microorganisms, we intraperitoneally injected blunt snout bream with A. hydrophila to explore the immune mechanism of socs3s. These results suggested that socs3s of blunt snout bream plays important roles in the regulation of embryonic development and tissue growth, and that socs3s may also play key roles in regulating the bacterial-induced congenital immune response. Socs3s genes has the potential to be used as targeted genes to improve the immunity against bacteria, which is conducive to the improvement of production and breeding.
中文翻译：
鉴定钝嘴鲷（Megalobrama amblycephala）中细胞因子信号传导3（SOCS3）基因的重复抑制子。
细胞因子信号传导抑制因子3（SOCS3）负调节各种免疫细胞因子的反应。在这项研究中，我们确定了钝嘴鲷的socs3s基因。209-aa和216-aa长的肽分别由socs3a和socs3b基因编码。socs3s mRNA在整个胚胎发育过程中始终表达。整座原位杂交在受精后12 h（hpf）检测到眼睛和后牙节中的socs3a，以24 hpf在耳囊上转录，然后以36 hpf在眼，脑和耳囊上转录。而socs3b mRNA则以12 hpf的速度在脊索处转录，以24 hpf的速度在脑，眼和尾巴中表达，并以36 hpf的速度在大脑中被检测到。在少年和成年钝嘴鲷的组织中，socs3a的表达与socs3b的表达略有不同。重组人生长激素（hGH）处理后，g，脾脏，肾脏和性腺中钝嘴鲷的socs3s的转录水平增加。嗜水气单胞菌感染后，在肝脏，脾脏，肠和肾组织中钝性鼻鲷的socs3的mRNA水平显着增加。钝嘴鲷对各种病原微生物均敏感，我们腹膜内注射钝嘴鲷与嗜水气单胞菌，以研究SOCS3s的免疫机制。这些结果表明钝嘴鲷的SOCS3在调节胚胎发育和组织生长中起重要作用，并且SOCS3在调节细菌诱导的先天性免疫应答中也可能起关键作用。Socs3s基因有可能被用作靶向基因，以提高针对细菌的免疫力，
更新日期：2019-09-14
中文翻译：
鉴定钝嘴鲷（Megalobrama amblycephala）中细胞因子信号传导3（SOCS3）基因的重复抑制子。
细胞因子信号传导抑制因子3（SOCS3）负调节各种免疫细胞因子的反应。在这项研究中，我们确定了钝嘴鲷的socs3s基因。209-aa和216-aa长的肽分别由socs3a和socs3b基因编码。socs3s mRNA在整个胚胎发育过程中始终表达。整座原位杂交在受精后12 h（hpf）检测到眼睛和后牙节中的socs3a，以24 hpf在耳囊上转录，然后以36 hpf在眼，脑和耳囊上转录。而socs3b mRNA则以12 hpf的速度在脊索处转录，以24 hpf的速度在脑，眼和尾巴中表达，并以36 hpf的速度在大脑中被检测到。在少年和成年钝嘴鲷的组织中，socs3a的表达与socs3b的表达略有不同。重组人生长激素（hGH）处理后，g，脾脏，肾脏和性腺中钝嘴鲷的socs3s的转录水平增加。嗜水气单胞菌感染后，在肝脏，脾脏，肠和肾组织中钝性鼻鲷的socs3的mRNA水平显着增加。钝嘴鲷对各种病原微生物均敏感，我们腹膜内注射钝嘴鲷与嗜水气单胞菌，以研究SOCS3s的免疫机制。这些结果表明钝嘴鲷的SOCS3在调节胚胎发育和组织生长中起重要作用，并且SOCS3在调节细菌诱导的先天性免疫应答中也可能起关键作用。Socs3s基因有可能被用作靶向基因，以提高针对细菌的免疫力，
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