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Daily variation of D2 dopamine receptor transcription in the brain of the Japanese eel Anguilla japonica and its regulation with dopamine and melatonin.
Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology ( IF 2.1 ) Pub Date : 2019-10-18 , DOI: 10.1016/j.cbpa.2019.110581 Jun-Hwan Byun 1 , Ji-Yeon Hyeon 2 , Eun-Su Kim 3 , Shin-Kwon Kim 4 , Sung-Pyo Hur 2 , Se-Jae Kim 5 , Akihiro Takemura 6
Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology ( IF 2.1 ) Pub Date : 2019-10-18 , DOI: 10.1016/j.cbpa.2019.110581 Jun-Hwan Byun 1 , Ji-Yeon Hyeon 2 , Eun-Su Kim 3 , Shin-Kwon Kim 4 , Sung-Pyo Hur 2 , Se-Jae Kim 5 , Akihiro Takemura 6
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Dopamine plays a crucial role in controlling reproduction in eels, and its action is mediated through D2-type dopamine receptors. D2A and D2B receptors in the Japanese eel Anguilla japonica were cloned and characterized in the present study. Attention (daily expression patterns in the brain and endogenous regulation) was paid to D2B receptor because it is considered to play a crucial role in eel reproduction. The cDNAs of D2A and D2B receptors had open reading frames comprising 456 and 454 amino acid residues, respectively, which were phylogenetically clustered with those of other teleost species. Both receptors were highly expressed in the brain. D2B receptor transcript levels exhibited high day/low night variation in the midbrain and pituitary, suggesting that its transcription in these tissues is regulated in a daily manner, possibly under influence of melatonin. Intraperitoneal injection of dopamine downregulated D2B receptor transcription significantly in the midbrain and moderately in the pituitary within 1 h, but upregulated its transcription in the forebrain. Co-injection of dopamine with its antagonist (domperidone) reversed the effect of dopamine in the pituitary and forebrain, but not in the midbrain, suggesting that the effect of dopamine on D2B receptor transcription differs among brain regions. The same treatment with melatonin resulted in decreased D2B receptor transcription in the midbrain. These findings indicate that dopamine and melatonin have key roles in the daily variation in D2B receptor transcription in the brain of Japanese eel, and that they are related to a daily base secretion of hormones in the hypothalamic-pituitary-gonadal axis in this species.
中文翻译：
日本鳗鳗大脑中D2多巴胺受体转录的每日变化及其对多巴胺和褪黑激素的调节。
多巴胺在控制鳗鱼繁殖中起着至关重要的作用，其作用是通过D2型多巴胺受体介导的。在本研究中克隆并鉴定了日本鳗鳗的D2A和D2B受体。注意（D2B受体在大脑中的日常表达方式和内源性调节）受到关注，因为它被认为在鳗鱼繁殖中起着至关重要的作用。D2A和D2B受体的cDNA具有分别包含456和454个氨基酸残基的开放阅读框，它们与其他硬骨鱼种在系统发育上集群在一起。两种受体均在脑中高表达。D2B受体的转录水平在中脑和垂体中表现出高昼夜变化，表明其在这些组织中的转录每天都受到调节，可能在褪黑激素的影响下。腹腔注射多巴胺可在1 h内显着下调脑中D2B受体的转录，并在垂体中下调D2B受体的转录，但在前脑中则上调D2B受体的转录。多巴胺与其拮抗剂（多潘立酮）的共同注射逆转了脑垂体和前脑中多巴胺的作用，但在中脑中却没有，这表明多巴胺对D2B受体转录的作用在大脑区域之间是不同的。褪黑素的相同处理导致中脑D2B受体转录降低。这些发现表明，多巴胺和褪黑激素在日本鳗鱼大脑中D2B受体转录的每日变化中起关键作用，
更新日期：2019-10-18
中文翻译：
日本鳗鳗大脑中D2多巴胺受体转录的每日变化及其对多巴胺和褪黑激素的调节。
多巴胺在控制鳗鱼繁殖中起着至关重要的作用，其作用是通过D2型多巴胺受体介导的。在本研究中克隆并鉴定了日本鳗鳗的D2A和D2B受体。注意（D2B受体在大脑中的日常表达方式和内源性调节）受到关注，因为它被认为在鳗鱼繁殖中起着至关重要的作用。D2A和D2B受体的cDNA具有分别包含456和454个氨基酸残基的开放阅读框，它们与其他硬骨鱼种在系统发育上集群在一起。两种受体均在脑中高表达。D2B受体的转录水平在中脑和垂体中表现出高昼夜变化，表明其在这些组织中的转录每天都受到调节，可能在褪黑激素的影响下。腹腔注射多巴胺可在1 h内显着下调脑中D2B受体的转录，并在垂体中下调D2B受体的转录，但在前脑中则上调D2B受体的转录。多巴胺与其拮抗剂（多潘立酮）的共同注射逆转了脑垂体和前脑中多巴胺的作用，但在中脑中却没有，这表明多巴胺对D2B受体转录的作用在大脑区域之间是不同的。褪黑素的相同处理导致中脑D2B受体转录降低。这些发现表明，多巴胺和褪黑激素在日本鳗鱼大脑中D2B受体转录的每日变化中起关键作用，
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