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Transcriptome profiling of the low-salinity stress responses in the gills of the juvenile Pseudopleuronectes yokohamae.
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.2 ) Pub Date : 2019-07-26 , DOI: 10.1016/j.cbd.2019.100612 Qianjin Cui 1 , Lihua Qiu 2 , Xu Yang 1 , Shengnan Shang 1 , Boxue Yang 1 , Mingkang Chen 1 , Xia Liu 1 , Bing Chen 1 , Xu Fu 1 , Wei Wang 1 , Chen Jiang 1
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.2 ) Pub Date : 2019-07-26 , DOI: 10.1016/j.cbd.2019.100612 Qianjin Cui 1 , Lihua Qiu 2 , Xu Yang 1 , Shengnan Shang 1 , Boxue Yang 1 , Mingkang Chen 1 , Xia Liu 1 , Bing Chen 1 , Xu Fu 1 , Wei Wang 1 , Chen Jiang 1
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Salinity is an important environmental factor that affects the life cycle of fish, including their growth, development and reproduction. The marbled flounder, Pseudopleuronectes yokohamae, is an important economic resource and serves as a good model to investigate osmoregulation, as it can adapt to a wide range of salinity levels. However, the lack of genomic resources for this species has hampered the understanding of the mechanisms underlying its salinity tolerance. In this study, RNA-Seq analysis was conducted to identify genes related to salinity adaptation and osmotic regulation in the gill tissue of marbled flounder exposed to different concentrations of environmental salinity (6 and 30 ppt). After de novo assembly, 19,265 genes were annotated by the Nr database. A comparison of expression between the two salinity groups revealed 673 differentially expressed genes, of which 180 were upregulated and 493 were downregulated in the low salinity group relative to the high salinity group. The related molecular biological processes were explored from several important perspectives, and potential functions were determined by enrichment analyses, including those of metabolites in ion transportation, energy metabolism and protein synthesis, and immune responses. This study is the first transcriptomic study conducted on marbled flounder, and it revealed many novel sequences for further biological analyses. In addition, the candidate genes identified in the gene expression analysis provided insights into responses to salinity change and molecular mechanisms underlying osmoregulation in the gills of marbled flounder.
中文翻译：
少年假横滨es的low中低盐度胁迫反应的转录组分析。
盐度是影响鱼类生命周期（包括其生长，发育和繁殖）的重要环境因素。大理石比目鱼（Pseudopleuronectes yokohamae）是重要的经济资源，是研究渗透压调节的良好模型，因为它可以适应多种盐度水平。然而，该物种缺乏基因组资源阻碍了对其耐盐性机制的理解。在这项研究中，进行了RNA-Seq分析，以鉴定与暴露于不同环境盐度（6和30 ppt）的比目鱼the组织中的盐度适应和渗透调节有关的基因。从头组装后，Nr数据库注释了19,265个基因。比较两个盐度组之间的表达，发现了673个差异表达基因，相对于高盐度组，低盐度组中有180个基因上调，而493个盐度下调。从几个重要的角度探讨了相关的分子生物学过程，并通过富集分析确定了潜在的功能，包括离子传递，能量代谢和蛋白质合成以及免疫反应中代谢物的富集分析。这项研究是对大理石比目鱼进行的第一个转录组学研究，它揭示了许多新颖的序列，可用于进一步的生物学分析。此外，在基因表达分析中鉴定出的候选基因为洞察盐分变化的反应以及大理石比目鱼the中渗透压调节的分子机制提供了见识。
更新日期：2019-07-26
中文翻译：
少年假横滨es的low中低盐度胁迫反应的转录组分析。
盐度是影响鱼类生命周期（包括其生长，发育和繁殖）的重要环境因素。大理石比目鱼（Pseudopleuronectes yokohamae）是重要的经济资源，是研究渗透压调节的良好模型，因为它可以适应多种盐度水平。然而，该物种缺乏基因组资源阻碍了对其耐盐性机制的理解。在这项研究中，进行了RNA-Seq分析，以鉴定与暴露于不同环境盐度（6和30 ppt）的比目鱼the组织中的盐度适应和渗透调节有关的基因。从头组装后，Nr数据库注释了19,265个基因。比较两个盐度组之间的表达，发现了673个差异表达基因，相对于高盐度组，低盐度组中有180个基因上调，而493个盐度下调。从几个重要的角度探讨了相关的分子生物学过程，并通过富集分析确定了潜在的功能，包括离子传递，能量代谢和蛋白质合成以及免疫反应中代谢物的富集分析。这项研究是对大理石比目鱼进行的第一个转录组学研究，它揭示了许多新颖的序列，可用于进一步的生物学分析。此外，在基因表达分析中鉴定出的候选基因为洞察盐分变化的反应以及大理石比目鱼the中渗透压调节的分子机制提供了见识。
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