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Diphenyl diselenide modulates splenic purinergic signaling in silver catfish fed diets contaminated with fumonisin B1: An attempt to improve immune and hemostatic responses.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2019-09-12 , DOI: 10.1016/j.cbpc.2019.108624 Matheus D Baldissera 1 , Carine F Souza 1 , Hugo Napoleão P da Silva 1 , Alessandra S Henn 2 , Fábio A Duarte 2 , Silvio T da Costa 3 , Aleksandro S Da Silva 4 , Bernardo Baldisserotto 1
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2019-09-12 , DOI: 10.1016/j.cbpc.2019.108624 Matheus D Baldissera 1 , Carine F Souza 1 , Hugo Napoleão P da Silva 1 , Alessandra S Henn 2 , Fábio A Duarte 2 , Silvio T da Costa 3 , Aleksandro S Da Silva 4 , Bernardo Baldisserotto 1
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The aim of this study was to determine whether purinergic signaling is a pathway associated with fumonisin B1 (FB1)-induced impairment of immune and hemostatic responses. We also determined whether dietary supplementation with diphenyl diselenide (Ph2Se2) prevents or reduces these effects. Splenic nucleoside triphosphate diphosphohydrolase (NTPDase) activity for adenosine triphosphate (ATP) and adenosine diphosphate (ADP) as substrates and total blood thrombocytes counts were significant lower in silver catfish fed with FB1-contaminated diets than in fish fed with a basal diet, while splenic adenosine deaminase (ADA) activity and metabolites of nitric oxide (NOx) levels were significant higher. Also, glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were significant lower in silver catfish fed with FB1-contaminated diets than in fish fed with a basal diet. Dietary supplementation with 3 mg Ph2Se2/kg of feed effectively modulated splenic NTPDase (ATP as substrate), ADA, GPx and SOD activities, as well as NOx levels, and was partially effective in the modulation of spleen NTPDase activity (ADP as substrate) and total blood thrombocytes count. These data suggest that splenic purinergic signaling of silver catfish fed with FB1-contaminated diets generates a pro-inflammatory profile that contributes to impairment of immune and inflammatory responses, via reduction of splenic ATP hydrolysis followed possible ATP accumulation in the extracellular environment. Reduction of ADP hydrolysis associated with possible accumulation in the extracellular environment can be a pathophysiological response that restricts the hemorrhagic process elicited by FB1 intoxication. Supplementation with Ph2Se2 effectively modulated splenic enzymes associated with control of extracellular nucleotides (except ADP; that was partially modulated) and nucleosides, thereby limiting inflammatory and hemorrhagic processes.
中文翻译：
二苯二硒化物可调节受伏马毒素B1污染的银silver鱼日粮中的脾嘌呤能传递信号：试图改善免疫和止血反应。
这项研究的目的是确定嘌呤能信号传导是否是与伏马菌素B1（FB1）诱导的免疫和止血反应损害相关的途径。我们还确定了饮食中补充二苯二硒（Ph2Se2）是否可以预防或减少这些影响。以三磷酸腺苷（ATP）和二磷酸腺苷（ADP）为底物的脾脏核苷三磷酸二磷酸水解酶（NTPDase）活性和以FB1污染的日粮喂养的银total鱼的总血细胞计数明显低于以基础饮食喂养的银silver鱼腺苷脱氨酶（ADA）活性和一氧化氮（NOx）代谢产物的含量显着较高。还，饲喂受FB1污染的日粮的银cat鱼中的谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的活性显着低于基础饮食的鱼。日粮中添加3 mg Ph2Se2 / kg饲料可有效调节脾脏NTPDase（以ATP为底物），ADA，GPx和SOD活性以及NOx含量，并且在调节脾脏NTPDase活性（以ADP为底物）方面部分有效。总血细胞计数。这些数据表明，饲喂受FB1污染的日粮喂养的银cat鱼的脾嘌呤能动信号产生了促炎特征，通过降低脾脏ATP水解以及可能在细胞外环境中积累ATP来降低免疫和炎症反应。与可能在细胞外环境中积累有关的ADP水解的减少可能是一种病理生理反应，它限制了FB1中毒引起的出血过程。补充Ph2Se2可有效调节与控制细胞外核苷酸（ADP除外；已部分调节）和核苷有关的脾脏酶，从而限制了炎症和出血过程。
更新日期：2019-09-12
中文翻译：
二苯二硒化物可调节受伏马毒素B1污染的银silver鱼日粮中的脾嘌呤能传递信号：试图改善免疫和止血反应。
这项研究的目的是确定嘌呤能信号传导是否是与伏马菌素B1（FB1）诱导的免疫和止血反应损害相关的途径。我们还确定了饮食中补充二苯二硒（Ph2Se2）是否可以预防或减少这些影响。以三磷酸腺苷（ATP）和二磷酸腺苷（ADP）为底物的脾脏核苷三磷酸二磷酸水解酶（NTPDase）活性和以FB1污染的日粮喂养的银total鱼的总血细胞计数明显低于以基础饮食喂养的银silver鱼腺苷脱氨酶（ADA）活性和一氧化氮（NOx）代谢产物的含量显着较高。还，饲喂受FB1污染的日粮的银cat鱼中的谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的活性显着低于基础饮食的鱼。日粮中添加3 mg Ph2Se2 / kg饲料可有效调节脾脏NTPDase（以ATP为底物），ADA，GPx和SOD活性以及NOx含量，并且在调节脾脏NTPDase活性（以ADP为底物）方面部分有效。总血细胞计数。这些数据表明，饲喂受FB1污染的日粮喂养的银cat鱼的脾嘌呤能动信号产生了促炎特征，通过降低脾脏ATP水解以及可能在细胞外环境中积累ATP来降低免疫和炎症反应。与可能在细胞外环境中积累有关的ADP水解的减少可能是一种病理生理反应，它限制了FB1中毒引起的出血过程。补充Ph2Se2可有效调节与控制细胞外核苷酸（ADP除外；已部分调节）和核苷有关的脾脏酶，从而限制了炎症和出血过程。
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