Label-free liquid biopsy based on blood circulating DNA detection using SERS-based nanotechnology for nasopharyngeal cancer screening.,Nanomedicine: Nanotechnology, Biology and Medicine

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Label-free liquid biopsy based on blood circulating DNA detection using SERS-based nanotechnology for nasopharyngeal cancer screening.
Nanomedicine: Nanotechnology, Biology and Medicine ( IF 4.2 ) Pub Date : 2019-10-21 , DOI: 10.1016/j.nano.2019.102100
Duo Lin ₁ , Qiong Wu ₂ , Sufang Qiu ₃ , Guannan Chen ₂ , Shangyuan Feng ₂ , Rong Chen ₂ , Haishan Zeng ₄

Affiliation

Development of a sensitive, rapid and easy-to-use liquid biopsy method is of imperative clinical value for point-of-care caner diagnostics. Here, a label-free and modification-free nanotechnology based on surface-enhanced Raman spectroscopy (SERS) was employed for DNA analysis. Using the SERS signals of phosphate backbone as internal standard, quantitative detection for nucleobases was achieved even at single base level. The method combined with principal component analysis and linear discriminant analysis was further applied for real blood circulating DNA detection for the first time, and an ideal diagnostic sensitivity of 83.3% and specificity of 82.5% could be obtained for differentiating the nasopharyngeal cancer from the normal group, demonstrating promising potential as an alternative nanotechnology for nasopharyngeal cancer screening based on liquid biopsy.

中文翻译：

基于血液循环DNA检测的无标记液体活检，使用基于SERS的纳米技术筛查鼻咽癌。

灵敏，快速且易于使用的液体活检方法的开发对于即时医疗诊断非常重要。在这里，基于表面增强拉曼光谱（SERS）的无标记和无修饰的纳米技术被用于DNA分析。使用磷酸盐主链的SERS信号作为内标，即使在单个碱基水平上，也可以实现对核碱基的定量检测。首次将主成分分析和线性判别分析相结合的方法首次用于真实血液循环DNA检测，可将鼻咽癌与正常组鉴别的理想诊断灵敏度为83.3％，特异性为82.5％。，

更新日期：2019-10-21

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