Gene Editing to Generate Versatile Human Pluripotent Stem Cell Reporter Lines for Analysis of Differentiation and Lineage Tracing,STEM CELLS

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Gene Editing to Generate Versatile Human Pluripotent Stem Cell Reporter Lines for Analysis of Differentiation and Lineage Tracing
STEM CELLS ( IF 4.0 ) Pub Date : 2019-11-13 , DOI: 10.1002/stem.3096
Xiaoping Bao _{1,

2,

3} , Maroof M Adil _{1,

2} , Riya Muckom _{1,

2} , Joshua A Zimmermann _{1,

2} , Aurelie Tran ₄ , Natalie Suhy ₄ , Yibo Xu ₃ , Rocío G Sampayo _{1,

2} , Douglas S Clark _{2,

5} , David V Schaffer _{1,

2,

3,

4}

Affiliation

Transcription factors (TFs) are potent proteins that control gene expression and can thereby drive cell fate decisions. Fluorescent reporters have been broadly knocked into endogenous TF loci to investigate the biological roles of these factors; however, the sensitivity of such analyses in human pluripotent stem cells (hPSCs) is often compromised by low TF expression levels and/or reporter silencing. Complementarily, we report an inducible and quantitative reporter platform based on the Cre‐LoxP recombination system that enables robust, quantifiable, and continuous monitoring of live hPSCs and their progeny to investigate the roles of TFs during human development and disease. Stem Cells 2019;37:1556–1566

中文翻译：

基因编辑生成多功能人类多能干细胞报告系，用于分化分析和谱系追踪

转录因子 (TF) 是控制基因表达的有效蛋白质，从而可以驱动细胞命运决定。荧光报告基因已被广泛敲入内源性 TF 基因座以研究这些因素的生物学作用；然而，在人类多能干细胞 (hPSC) 中进行此类分析的灵敏度通常会受到低 TF 表达水平和/或报告基因沉默的影响。作为补充，我们报告了一个基于 Cre-LoxP 重组系统的可诱导和定量报告平台，该平台能够对活 hPSC 及其后代进行稳健、可量化和持续的监测，以研究 TF 在人类发育和疾病中的作用。干细胞 2019；37：1556–1566

更新日期：2019-11-13

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