当前位置: X-MOL 学术STEM CELLS › 论文详情
Gene Editing to Generate Versatile Human Pluripotent Stem Cell Reporter Lines for Analysis of Differentiation and Lineage Tracing.
STEM CELLS ( IF 6.022 ) Pub Date : 2019-11-13 , DOI: 10.1002/stem.3096
Xiaoping Bao,Maroof M Adil,Riya Muckom,Joshua A Zimmermann,Aurelie Tran,Natalie Suhy,Yibo Xu,Rocío G Sampayo,Douglas S Clark,David V Schaffer

Transcription factors (TFs) are potent proteins that control gene expression and can thereby drive cell fate decisions. Fluorescent reporters have been broadly knocked into endogenous TF loci to investigate the biological roles of these factors; however, the sensitivity of such analyses in human pluripotent stem cells (hPSCs) is often compromised by low TF expression levels and/or reporter silencing. Complementarily, we report an inducible and quantitative reporter platform based on the Cre-LoxP recombination system that enables robust, quantifiable, and continuous monitoring of live hPSCs and their progeny to investigate the roles of TFs during human development and disease. Stem Cells 2019;37:1556-1566.
更新日期:2019-11-13

 

全部期刊列表>>
材料学研究精选
Springer Nature Live 产业与创新线上学术论坛
胸腔和胸部成像专题
自然科研论文编辑服务
ACS ES&T Engineering
ACS ES&T Water
屿渡论文,编辑服务
杨超勇
周一歌
华东师范大学
南京工业大学
清华大学
中科大
唐勇
跟Nature、Science文章学绘图
隐藏1h前已浏览文章
中洪博元
课题组网站
新版X-MOL期刊搜索和高级搜索功能介绍
ACS材料视界
x-mol收录
福州大学
南京大学
王杰
左智伟
湖南大学
清华大学
吴杰
赵延川
中山大学化学工程与技术学院
试剂库存
天合科研
down
wechat
bug