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In vitro digestion method to evaluate solubility of dietary zinc, selenium and manganese in salmonid diets.
Journal of Trace Elements in Medicine and Biology ( IF 3.6 ) Pub Date : 2019-10-20 , DOI: 10.1016/j.jtemb.2019.126418 Marta S Silva 1 , P Antony Jesu Prabhu 2 , Robin Ørnsrud 2 , Veronika Sele 2 , Saskia Kröckel 3 , Jens J Sloth 4 , Heidi Amlund 5
Journal of Trace Elements in Medicine and Biology ( IF 3.6 ) Pub Date : 2019-10-20 , DOI: 10.1016/j.jtemb.2019.126418 Marta S Silva 1 , P Antony Jesu Prabhu 2 , Robin Ørnsrud 2 , Veronika Sele 2 , Saskia Kröckel 3 , Jens J Sloth 4 , Heidi Amlund 5
Affiliation
BACKGROUND
The determination of dietary mineral solubility is one of the main steps in the evaluation of their availability for a given species.
METHODS
This study proposed an in vitro digestion method (acidic and alkaline hydrolysis). The method was applied to evaluate the solubility of inorganic and organic forms of zinc (Zn), selenium (Se) and manganese (Mn) in salmonid diets. An inorganic mineral (IM) diet was supplemented with zinc sulphate, sodium selenite and manganous sulphate and an organic mineral (OM) diet was supplemented with zinc chelate of glycine, l-selenomethionine and manganese chelate of glycine.
RESULTS
The solubility of Zn was similar in both diets tested. The amount of soluble Zn was low in the acidic hydrolysis (3-8%) and lower in the alkaline hydrolysis (0.4-2%). The solubility of Se was higher in the OM diet (7-34%) compared with the IM diet (3-12%). Regarding Mn, after the acidic hydrolysis the solubility was higher in the IM diet (6-25%) than the OM diet (4-17%). The in vitro solubility were compared with in vivo availability of Zn, Se and Mn. Data obtained for solubility (%) of Zn, Se and Mn was lower when compared with apparent availability (%) of Zn, Se and Mn.
CONCLUSION
Data obtained demonstrated that solubility of Zn, Se and Mn was influenced by the mineral chemical form supplemented to the diet and by the gastrointestinal environment. The solubility of Zn, Se and Mn was not comparable with the apparent availability of Zn, Se and Mn. Nevertheless, the effect of the chemical form of the minerals was similar for the solubility of Zn, Se and Mn and the apparent availability of Zn, Se and Mn. Considering the overall results of this study, the in vitro method could replace some of the in vivo studies for a qualitative evaluation but not for a quantitative evaluation.
中文翻译：
体外消化法评估鲑鱼日粮中锌，硒和锰的溶解度。
背景技术膳食矿物质溶解度的确定是评估给定物种的矿物质溶解度的主要步骤之一。方法本研究提出了一种体外消化方法（酸性和碱性水解）。该方法用于评估鲑鱼日粮中无机和有机形式的锌（Zn），硒（Se）和锰（Mn）的溶解度。无机矿物质（IM）饮食中补充有硫酸锌，亚硒酸钠和硫酸锰，有机矿物质（OM）饮食中补充有甘氨酸的锌螯合物，1-硒代蛋氨酸和甘氨酸的锰螯合物。结果两种测试日粮中锌的溶解度相似。可溶性Zn的量在酸性水解中较低（3-8％），而在碱性水解中较低（0.4-2％）。与IM饮食（3-12％）相比，OM饮食（7-34％）中Se的溶解度更高。关于Mn，在酸性水解之后，IM日粮中的溶解度（6-25％）高于OM日粮中的溶解度（4-17％）。将体外溶解度与体内锌，硒和锰的利用率进行了比较。与Zn，Se和Mn的表观利用率（％）相比，获得的Zn，Se和Mn溶解度（％）数据较低。结论获得的数据表明，锌，硒和锰的溶解度受日粮中添加的矿物化学形式和胃肠道环境的影响。Zn，Se和Mn的溶解度无法与Zn，Se和Mn的表观可用性相提并论。然而，矿物质化学形式的影响对于Zn，Se和Mn的溶解度以及Zn，Se和Mn的表观可用性是相似的。
更新日期：2019-10-21
中文翻译：
体外消化法评估鲑鱼日粮中锌，硒和锰的溶解度。
背景技术膳食矿物质溶解度的确定是评估给定物种的矿物质溶解度的主要步骤之一。方法本研究提出了一种体外消化方法（酸性和碱性水解）。该方法用于评估鲑鱼日粮中无机和有机形式的锌（Zn），硒（Se）和锰（Mn）的溶解度。无机矿物质（IM）饮食中补充有硫酸锌，亚硒酸钠和硫酸锰，有机矿物质（OM）饮食中补充有甘氨酸的锌螯合物，1-硒代蛋氨酸和甘氨酸的锰螯合物。结果两种测试日粮中锌的溶解度相似。可溶性Zn的量在酸性水解中较低（3-8％），而在碱性水解中较低（0.4-2％）。与IM饮食（3-12％）相比，OM饮食（7-34％）中Se的溶解度更高。关于Mn，在酸性水解之后，IM日粮中的溶解度（6-25％）高于OM日粮中的溶解度（4-17％）。将体外溶解度与体内锌，硒和锰的利用率进行了比较。与Zn，Se和Mn的表观利用率（％）相比，获得的Zn，Se和Mn溶解度（％）数据较低。结论获得的数据表明，锌，硒和锰的溶解度受日粮中添加的矿物化学形式和胃肠道环境的影响。Zn，Se和Mn的溶解度无法与Zn，Se和Mn的表观可用性相提并论。然而，矿物质化学形式的影响对于Zn，Se和Mn的溶解度以及Zn，Se和Mn的表观可用性是相似的。
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