Field‐effect transistor (FET) biosensors based on hybrid nanostructures present unique advantages of high sensitivity, small size, excellent dynamic range, and compatibility with integrated circuits. In this work, the fabrication of highly sensitive and selective hybrid MoS₂/TiO₂ nanostructure‐based FET is demonstrated for the detection of Gram‐positive bacteria. The fabricated FET biosensors are efficiently capable of discriminating between Gram‐positive and Gram‐negative bacteria with a limit of detection 50 cfu mL⁻¹. The biosensor exhibits a high sensitivity of 49.47 ± 1.7% for Staphylococcus aureus among eight different bacteria at a concentration of 10² cfu mL⁻¹. The real time detection of bacteria with a response time of 22.19 s is achieved at concentrations as low as 50–100 cfu mL⁻¹, suggesting rapid and low concentration detection of selective bacteria. The FET biosensor is also able to quantify bacteria in the physiological conditions as well as discriminate between live and dead bacteria. The unique hybrid nanostructure based FET with desirable biosensing properties presents the potential to screen the pathogenic bacteria. Live/dead monitoring of bacteria may help in deciding the course and dosage of the antibiotic treatment.

中文翻译：

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基于MoS2 / TiO2杂化纳米结构的场效应晶体管，用于高灵敏度，选择性和快速检测革兰氏阳性细菌

基于混合纳米结构的场效应晶体管（FET）生物传感器具有高灵敏度，小尺寸，出色的动态范围以及与集成电路兼容的独特优势。在这项工作中，展示了用于检测革兰氏阳性细菌的高敏感度和选择性的基于MoS ₂ / TiO ₂纳米结构的混合FET的制造。所制造的FET生物传感器能够有效区分革兰氏阳性菌和革兰氏阴性菌，检出限为50 cfu mL ^-1。该生物传感器在10 ² cfu mL ^-1的浓度下对八种不同细菌中的金黄色葡萄球菌表现出49.47±1.7％的高灵敏度。在低至50–100 cfu mL ^-1的浓度下，即可实时检测到细菌的响应时间为22.19 s ，这表明对选择性细菌的检测速度低而又低。FET生物传感器还能够对生理条件下的细菌进行定量，并区分活细菌和死细菌。具有所需生物传感特性的独特的基于混合纳米结构的FET具有筛选病原菌的潜力。细菌的生/死监测可能有助于确定抗生素治疗的过程和剂量。