The sucrose non-fermenting-1-related protein kinase 1 (SnRK1) is a highly conserved heterotrimeric protein kinase in plants. It possesses a catalytic subunit (α) and two regulatory subunits (β and γ). The effects of altered expression of AKINβ1 on carbohydrate metabolism and gene expression in leaves were investigated in an Arabidopsis T-DNA insertion mutant. The contents of key intermediates in the tricarboxylic acid (TCA) cycle of the mutant leaves were markedly reduced throughout the diurnal cycle, coupled with a decrease in measurable respiration rate. Compared with the wild type, 2485 genes and 188 genes were differentially expressed in leaves of the akinβ1 mutant in response to light and darkness, respectively. Among these, several genes exhibited very substantial decreases in expression. Notably, expression of particular isoforms of multigene families involved in malate and lipid metabolism and nitrate uptake showed decreases of 40- to 240-fold during the light period, but not during darkness. The subcellular localization of AKINβ1 and the regulatory function of N-myristoylation for this localization were investigated, showing that AKINβ1 localizes to the Golgi. A model is hypothesized to explain the effects of AKINβ1 on metabolism and gene expression in Arabidopsis.

中文翻译：

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ARKβ1是SnRK1的一个亚基，它调节有机酸代谢，并充当涉及碳，脂质和氮代谢的基因的整体调节剂。

蔗糖非发酵-1相关蛋白激酶1（SnRK1）是植物中高度保守的异三聚体蛋白激酶。它具有一个催化亚基（α）和两个调节亚基（β和γ）。在拟南芥T-DNA插入突变体中，研究了AKINβ1表达改变对叶片碳水化合物代谢和基因表达的影响。在整个昼夜循环中，突变叶片的三羧酸（TCA）循环中关键中间体的含量均显着降低，同时可测量的呼吸速率降低。与野生型相比，akinβ1突变体的叶片分别响应于光照和黑暗而差异表达了2485个基因和188个基因。在这些基因中，几个基因表现出非常显着的表达下降。尤其，苹果酸，脂质代谢和硝酸盐吸收的多基因家族特定同工型的表达在光亮时期减少了40到240倍，而在黑暗时期则没有。研究了AKINβ1的亚细胞定位和N-肉豆蔻酰化对该定位的调节功能，表明AKINβ1定位于高尔基体。假设有一个模型来解释AKINβ1对拟南芥代谢和基因表达的影响。