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Multioside, an active ingredient from adonis amurensis, displays anti-cancer activity through autophagosome formation.
Phytomedicine ( IF 6.7 ) Pub Date : 2019-10-19 , DOI: 10.1016/j.phymed.2019.153114 Dae Won Park 1 , Young Min Ham 2 , Yeong-Geun Lee 1 , Rina So 1 , Young-Jin Seo 3 , Se Chan Kang 1
Phytomedicine ( IF 6.7 ) Pub Date : 2019-10-19 , DOI: 10.1016/j.phymed.2019.153114 Dae Won Park 1 , Young Min Ham 2 , Yeong-Geun Lee 1 , Rina So 1 , Young-Jin Seo 3 , Se Chan Kang 1
Affiliation
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BACKGROUND
Adonis amurensis Regel & Radde, commonly found in East Asia, has been traditionally used to treat cardiac insufficiency and edema. Although this plant extract has been shown to regulate cell growth and neovascularization, the anti-cancer mechanism of A. amurensis has not been fully investigated.
PURPOSE
In this study, we aimed to examine the anti-cancer activity of A. amurensis and identify its underlying mechanism.
METHODS
The growth of cancer cells was evaluated by MTT and hollow fiber assays. A cancer xenograft nude mouse model was used to assess the anti-cancer activities in vivo. Autophagic activity was measured by the detection of autophagosome formation and by performing a monodansylcadaverine (MDC) assay.
RESULT
A. amurensis extract showed potent anti-cancer activity both in vitro and in vivo. Importantly, the treatment of cancer cells with A. amurensis extract dramatically increased the formation of autophagosomes and was involved in the activation of multiple signaling components including AKT, ERK, and MAPK. Furthermore, we isolated an active ingredient, Multioside, which exhibited strong anti-cancer activity through autophagy.
CONCLUSION
A. amurensis displays anti-cancer activity that is mediated by the activation of autophagy, suggesting that A. amurensis could be a useful therapeutic anti-cancer agent.
中文翻译：
Multioside是一种来自阿多尼斯amurensis的活性成分，它通过自噬小体的形成显示出抗癌活性。
背景技术通常在东亚发现的Adonis amurensis Regel＆Radde传统上已用于治疗心脏功能不全和水肿。尽管已表明该植物提取物可调节细胞生长和新血管形成，但尚未完全研究紫mur曲霉的抗癌机制。目的在这项研究中，我们旨在检查A. amurensis的抗癌活性并确定其潜在机制。方法通过MTT和中空纤维测定法评估癌细胞的生长。癌症异种移植裸鼠模型用于评估体内的抗癌活性。通过检测自噬体的形成并通过进行单丹磺酰尸胺（MDC）测定来测量自噬活性。结果A. amurensis提取物在体外和体内均显示出有效的抗癌活性。重要的，用A. amurensis提取物处理癌细胞会大大增加自噬体的形成，并参与了包括AKT，ERK和MAPK在内的多种信号成分的激活。此外，我们分离了一种活性成分Multioside，它通过自噬表现出强大的抗癌活性。结论A. amurensis表现出抗癌活性，该活性由自噬激活介导，表明A. amurensis可能是有用的治疗性抗癌剂。
更新日期：2019-10-19
中文翻译：
Multioside是一种来自阿多尼斯amurensis的活性成分，它通过自噬小体的形成显示出抗癌活性。
背景技术通常在东亚发现的Adonis amurensis Regel＆Radde传统上已用于治疗心脏功能不全和水肿。尽管已表明该植物提取物可调节细胞生长和新血管形成，但尚未完全研究紫mur曲霉的抗癌机制。目的在这项研究中，我们旨在检查A. amurensis的抗癌活性并确定其潜在机制。方法通过MTT和中空纤维测定法评估癌细胞的生长。癌症异种移植裸鼠模型用于评估体内的抗癌活性。通过检测自噬体的形成并通过进行单丹磺酰尸胺（MDC）测定来测量自噬活性。结果A. amurensis提取物在体外和体内均显示出有效的抗癌活性。重要的，用A. amurensis提取物处理癌细胞会大大增加自噬体的形成，并参与了包括AKT，ERK和MAPK在内的多种信号成分的激活。此外，我们分离了一种活性成分Multioside，它通过自噬表现出强大的抗癌活性。结论A. amurensis表现出抗癌活性，该活性由自噬激活介导，表明A. amurensis可能是有用的治疗性抗癌剂。
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