Amino acids are vital components in cell metabolism. Leucine is a regulatory factor that generates significant impact on protein synthesis/turnover, modulates diverse cellular signalling pathways and participates in oxidative processes and immune responses. Here, we identified and characterized the functions of a leucine‐associated Zn₂Cys₆‐type transcription factor, MoLeu3. Disruption of MoLEU3 resulted in significantly reduced pathogenicity in barley and rice. Quantitative RT‐PCR showed that the expression levels of the putative leucine biosynthesis‐related genes, MoLEU1, MoLEU2 and MoLEU4 were downregulated in the ΔMoleu3 mutant. We used high‐throughput gene knockout method to generate the null mutants of MoLEU1, MoLEU2 and MoLEU4 respectively. The ΔMoleu1, ΔMoleu2 and ΔMoleu4 mutants are leucine auxotroph and showed similar phenotypic characterizations, including reduced conidiation, delayed mobilization and degradation of glycogen and lipid droplets, limited appressorium‐mediated penetration, and restricted invasive hyphae growth within host cells. Collectively, MoLEU1, MoLEU2, and MoLEU4 regulated by MoLEU3 play crucial roles in fungal development and infectious processes through modulation of leucine biosynthesis in Magnaporthe oryzae.

中文翻译：

---

受MoLEU3调控的MoLEU1，MoLEU2和MoLEU4与稻瘟病菌中的亮氨酸生物合成，真菌发育和致病性有关。

氨基酸是细胞代谢中的重要组成部分。亮氨酸是一种调节因子，可对蛋白质合成/周转产生重大影响，调节多种细胞信号传导途径，并参与氧化过程和免疫反应。在这里，我们确定并表征了亮氨酸相关的Zn ₂ Cys ₆型转录因子MoLeu3的功能。MoLEU3的破坏导致大麦和大米的致病性大大降低。定量RT-PCR表明，推定的亮氨酸生物合成的相关的基因的表达水平，MoLEU1，MoLEU2和MoLEU4在Δ被下调Moleu3突变体。我们使用高通量基因敲除方法分别生成MoLEU1，MoLEU2和MoLEU4的无效突变体。的Δ Moleu1，Δ Moleu2和Δ Moleu4突变体是亮氨酸营养缺陷型和表现出相似的表型表征，包括减少产孢，延迟动员和糖原和脂滴的降解，限制附着胞介导的渗透，和宿主细胞内限制侵入菌丝生长。总的来说，MoLEU1，MoLEU2和MoLEU4通过调节MoLEU3通过调节稻瘟病菌中亮氨酸的生物合成在真菌的发育和传染过程中发挥重要作用。