One-step photostructuring of multiple hydrogel arrays for compartmentalized enzyme reactions in microfluidic devices,Reaction Chemistry & Engineering

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One-step photostructuring of multiple hydrogel arrays for compartmentalized enzyme reactions in microfluidic devices
Reaction Chemistry & Engineering ( IF 3.4 ) Pub Date : 2019-10-17 , DOI: 10.1039/c9re00349e
Franziska Obst _{1,

2,

3,

4,

5} , David Simon _{1,

2,

3,

4,

5} , Philipp J. Mehner _{3,

4,

6,

7} , Jens W. Neubauer _{1,

2,

3} , Anthony Beck _{3,

4,

6,

7} , Oleksandr Stroyuk _{2,

3,

4,

8} , Andreas Richter _{3,

4,

6,

7} , Brigitte Voit _{1,

2,

3,

4,

5} , Dietmar Appelhans _{1,

2,

3}

Affiliation

We present a technique for the simultaneous photostructuring of hydrogels on the μm scale with different compositions on one substrate. The existing methods (e.g. spin-coating of the hydrogel precursor and application of simple moulds or spacers) have less potential to integrate spatially separated functional hydrogel dots as needed for the fabrication of complex polydimethylsiloxane (PDMS) on-glass microfluidic chips. Here, we report a method for the fast production of precisely placed hydrogel structures with adjustable height (≤80 μm) and width (≤500 μm) on glass substrates utilizing black polyoxymethylene moulds. The integration of several chambers for different hydrogel precursor solutions in one mould allows the simultaneous photopolymerization of hydrogels with different compositions. To visualize the difference in composition of the hydrogel dots and to demonstrate the precision in structuring, various quantum dots were added to the hydrogel precursor solutions and physically entrapped during the photopolymerization. To show the high potential of our photostructuring method, a microfluidic double-chamber reactor was designed and the variability in the performance of tri-enzymatic cascade reactions with optionally spatially separated or non-separated enzymes β-galactosidase, glucose oxidase, and horseradish peroxidase was visualized.

中文翻译：

用于微流控设备中间隔酶反应的多个水凝胶阵列的一步式光结构化

我们提出了一种技术，用于在一个基板上以不同的组成同时对微米级的水凝胶进行光结构化。现有方法（例如水凝胶前体的旋涂以及简单模具或垫片的应用，在玻璃上微流体芯片上制造复杂的聚二甲基硅氧烷（PDMS）时，整合空间分离的功能性水凝胶点的潜力较小。在这里，我们报告了一种利用黑色聚甲醛模具在玻璃基板上快速生产高度可调（≤80μm）和宽度（≤500μm）的精确放置水凝胶结构的方法。在一个模具中将多个用于不同水凝胶前体溶液的腔室整合在一起，可以使具有不同组成的水凝胶同时进行光聚合。为了可视化水凝胶点组成的差异并证明结构化的准确性，将各种量子点添加到水凝胶前体溶液中，并在光聚合过程中将其物理截留。为了显示我们的光结构化方法的巨大潜力，设计了一种微流控双腔反应器，并选择了在空间上分离或未分离的酶β-半乳糖苷酶，葡萄糖氧化酶和辣根过氧化物酶进行三酶级联反应的性能差异。可视化。

更新日期：2019-10-17

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