pH-Responsive protein nanoparticles via conjugation of degradable PEG to the surface of cytochrome c†,Polymer Chemistry

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pH-Responsive protein nanoparticles via conjugation of degradable PEG to the surface of cytochrome c†
Polymer Chemistry ( IF 4.1 ) Pub Date : 2019-10-17 , DOI: 10.1039/c9py01162e
Elena Steiert _{1,

2,

3,

4} , Johannes Ewald _{2,

3,

4,

5} , Annika Wagner _{1,

2,

3,

4} , Ute A. Hellmich _{1,

2,

3,

4} , Holger Frey _{2,

3,

4,

5} , Peter R. Wich _{1,

2,

3,

4,

6}

Affiliation

Proteins represent a versatile biopolymer material for the preparation of nanoparticles. For drug delivery applications an acid-triggered disassembly and payload release is preferred. Herein, we present a protein nanoparticle system based on cytochrome c, which is surface-modified with acid-degradable polyethylene glycol (PEGylation). pH-Sensitivity was obtained through vinyl ether moieties distributed in the polyether backbone. When PEGylated, cytochrome c shows a different solubility behaviour in organic solvents, which allows for particle preparation using an emulsion-based solvent evaporation method. The resulting particles are stable under physiological conditions but degrade at acidic pH values. Fluorescence-labelled dextran was successfully encapsulated as a hydrophilic model payload in these degradable nanoparticles and a release under acidic conditions was observed.

中文翻译：

pH-响应蛋白纳米颗粒通过降解将PEG缀合至细胞色素的表面Ç †

蛋白质代表了用于制备纳米粒子的通用生物聚合物材料。对于药物输送应用，优选酸触发的拆卸和有效载荷释放。在本文中，我们提出了一种基于细胞色素c的蛋白质纳米颗粒系统，该系统用酸可降解的聚乙二醇进行了表面修饰（聚乙二醇化）。通过分布在聚醚主链中的乙烯基醚部分获得pH敏感性。聚乙二醇化时，细胞色素c在有机溶剂中显示出不同的溶解性能，这允许使用基于乳液的溶剂蒸发方法制备颗粒。所得颗粒在生理条件下是稳定的，但在酸性pH值下会降解。荧光标记的葡聚糖已成功封装为这些可降解纳米颗粒中的亲水模型有效载荷，并观察到在酸性条件下的释放。

更新日期：2020-01-02

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