Autologous bone grafts constitute the second most transplanted tissue in medicine today. The viability, and consequently the osteogenic capacity, of an autograft is directly impacted by the interval between harvest and transplantation, but how the temperature and the solution in which the graft is held intraoperatively affect viability is not clear. Using a syngeneic mouse model and in vivo bone-forming assays, these variables were tested for their effects on programmed cell death, osteoprogenitor cell proliferation, and the ability of the autograft to ultimately produce new bone in an ectopic site. Based on these results, the intraoperative treatment with a WNT protein therapeutic was tested for its effects on the viability and osteogenic capacity of an autograft. Viability, programmed cell death, mitotic activity, osteogenic protein expression, and bone-forming capacity were assessed. Experimental results demonstrated that the osteogenic capacity of an autograft is significantly improved by intraoperative storage in L-WNT3A at physiological temperature.

中文翻译：

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改善自体骨移植的术中存储条件：在小鼠中进行的实验研究。

自体骨移植物构成当今医学上第二大移植组织。自体移植物的生存能力和因此的成骨能力直接受到收获和移植之间的时间间隔的影响，但是尚不清楚温度和保存移植物的溶液在术中如何影响生存能力。使用同系小鼠模型和体内成骨试验，测试了这些变量对程序性细胞死亡，骨祖细胞增殖和自体移植物最终在异位部位产生新骨的能力的影响。基于这些结果，测试了使用WNT蛋白治疗剂进行的术中治疗对自体移植物的生存能力和成骨能力的影响。生存力，程序性细胞死亡，有丝分裂活性，成骨蛋白表达，和骨形成能力进行了评估。实验结果表明，通过在生理温度下在L-WNT3A中进行术中存储，可以显着提高自体移植物的成骨能力。