The effect of green light on the motility of mouse sperm at two different temperatures.,Photochemical & Photobiological Sciences

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The effect of green light on the motility of mouse sperm at two different temperatures.
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2019-11-04 , DOI: 10.1039/c9pp00258h
M Ríos-Ramírez ₁ , J Horacio Espinoza ₂ , J C Ruiz-Suárez ₁ , H Mercado-Uribe ₁

Affiliation

Photo-modulation with visible light has been used to induce gains in the motility of the sperms of rabbits, boars, buffalo, bulls, fish, and humans. Although different hypotheses have been proposed to explain such an effect, the origin and mechanisms by which visible light affects sperm motility are still far from being completely understood. Several groups have observed changes in the intracellular Ca2+ concentration and significant differences in the production of ROS, which are attributed to specific photosensitizers. Also, it has been reported that blue light induces nitric oxide production in sperm cells, which plays a vital role in acrosome reaction and capacitation leading to an augmentation in the fertilisation probability. In the present work, we study the effects of green light (490-540 nm) on the sperm motility of mice. Firstly, we carried out experiments at 37 °C to confirm what previous researchers have observed before using red and blue light: that the overall sperm motility increases. Secondly, we studied the effects of green light at 10 °C and found that the motility drastically diminishes. In order to understand this opposing outcome, we carried out fluorescence measurements to evaluate reactive oxygen species production induced by green light at both temperatures. Our results suggest that the balance between the use and generation of ROS at 37 °C is favorable to the cells, while at 10 °C it is harmful.

中文翻译：

在两种不同温度下，绿光对小鼠精子运动的影响。

可见光的光调制已被用于诱导兔子，公猪，水牛，公牛，鱼和人的精子运动能力的提高。尽管提出了不同的假设来解释这种作用，但仍远没有完全理解可见光影响精子运动的起源和机理。几个小组已经观察到细胞内Ca2 +浓度的变化和ROS产生的显着差异，这归因于特定的光敏剂。另外，据报道，蓝光在精子细胞中诱导一氧化氮的产生，这在顶体反应和获能中起着至关重要的作用，从而导致受精率的增加。在目前的工作中，我们研究了绿色光（490-540 nm）对小鼠精子运动的影响。首先，我们在37°C的温度下进行了实验，以确认以前的研究人员在使用红光和蓝光之前观察到的内容：精子的整体运动力增加了。其次，我们研究了10°C的绿光的影响，发现运动性急剧降低。为了理解这种相反的结果，我们进行了荧光测量以评估在两个温度下由绿光诱导的活性氧的产生。我们的结果表明，在37°C下使用和生成ROS之间的平衡对细胞有利，而在10°C下则有害。为了理解这种相反的结果，我们进行了荧光测量以评估在两个温度下由绿光诱导的活性氧的产生。我们的结果表明，在37°C下使用和生成ROS之间的平衡对细胞有利，而在10°C下则有害。为了理解这种相反的结果，我们进行了荧光测量以评估在两个温度下由绿光诱导的活性氧的产生。我们的结果表明，在37°C下使用和生成ROS之间的平衡对细胞有利，而在10°C下则有害。

更新日期：2019-12-04

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