Selective recruitment of Nck and Syk contribute to distinct leukocyte immune-type receptor-initiated target interactions.,Cellular Signalling

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Selective recruitment of Nck and Syk contribute to distinct leukocyte immune-type receptor-initiated target interactions.
Cellular Signalling ( IF 4.4 ) Pub Date : 2019-10-15 , DOI: 10.1016/j.cellsig.2019.109443
Dustin M E Lillico ₁ , Joshua G Pemberton ₂ , Rikus Niemand ₁ , James L Stafford ₁

Affiliation

The ability of phagocytes to recognize, immobilize, and engulf extracellular targets are fundamental immune cell processes that allow for the destruction of a variety of microbial intruders. The phagocytic process depends onsignalling events that initiate dynamic changes in the plasma membrane architecture that are required to accommodate the internalization of large particulate targets. To better understand fundamental molecular mechanisms responsible for facilitating phagocytic receptor-mediated regulation of cytoskeletal networks, our research has focused on investigating representative immunoregulatory proteins from the channel catfish (Ictalurus punctatus) leukocyte immune-type receptor family (IpLITRs). Specifically, we have shown that a specific IpLITR-type can regulate the constitutive deployment of filopodial-like structures to actively capture and secure targets to the phagocyte surface, which is followed by F-actin mediated membrane dynamics that are associated with the formation of phagocytic cup-like structures that precede target engulfment. In the present study, we use confocal imaging to examine the recruitment of mediators of the F-actin cytoskeleton during IpLITR-mediated regulation of membrane dynamics. Our results provide novel details regarding the dynamic recruitment of the signaling effectors Nck and Syk during classical as well as atypical IpLITR-induced phagocytic processes.

中文翻译：

Nck和Syk的选择性募集有助于独特的白细胞免疫型受体启动的靶标相互作用。

吞噬细胞识别，固定和吞噬细胞外靶标的能力是基本的免疫细胞过程，可破坏多种微生物入侵者。吞噬过程取决于信号转导事件，该事件引发质膜结构的动态变化，而动态变化是适应大颗粒靶标内在化所必需的。为了更好地理解负责促进吞噬受体介导的细胞骨架网络调节的基本分子机制，我们的研究集中在研究来自cat鱼（Ictalurus punctatus）白细胞免疫型受体家族（IpLITRs）的代表性免疫调节蛋白。具体来说，我们已经证明，特定的IpLITR类型可以调节丝状动物结构的结构性部署，以主动捕获并将靶标固定在吞噬细胞表面，然后由F-肌动蛋白介导的与吞噬杯形成相关的膜动力学。如目标吞噬之前的结构。在本研究中，我们使用共聚焦成像来检查IpLITR介导的膜动力学调节过程中F-肌动蛋白细胞骨架的介体募集。我们的结果提供了有关经典以及非典型IpLITR诱导的吞噬过程中信号转导因子Nck和Syk动态募集的新颖细节。随后是F-肌动蛋白介导的膜动力学，该动力学与目标吞噬之前吞噬杯状结构的形成有关。在本研究中，我们使用共聚焦成像来检查IpLITR介导的膜动力学调节过程中F-肌动蛋白细胞骨架的介体募集。我们的结果提供了有关经典以及非典型IpLITR诱导的吞噬过程中信号转导因子Nck和Syk动态募集的新颖细节。随后是F-肌动蛋白介导的膜动力学，该动力学与目标吞噬之前吞噬杯状结构的形成有关。在本研究中，我们使用共聚焦成像来检查IpLITR介导的膜动力学调节过程中F-肌动蛋白细胞骨架的介体募集。我们的结果提供了有关经典以及非典型IpLITR诱导的吞噬过程中信号转导因子Nck和Syk动态募集的新颖细节。

更新日期：2019-11-18

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