INTRODUCTION Oral microflora is a well-orchestrated and acts as a sequential defense mechanism for any infection related to oral disease. Chronic periodontitis is a disease of a microbial challenge to symbiosis and homeostasis. Periodontal surgery is the most promising cure with repair process during periodontal regeneration. It has an encouraging outcome in terms of early recovery biomarkers. OBJECTIVE Saliva of periodontal surgery subjects with the chronic periodontitis have been evaluated by 1H NMR spectroscopy in search of possible early metabolic differences that could be obtained in order to see the eradication of disease which favours the symbiotic condition. METHOD The study employed 1H NMR spectroscopy on 176 human saliva samples in search of distinctive differences and their spectral data were further subjected to multivariate and quantitative analysis. RESULT The 1H NMR study of periodontal surgery samples shows clear demarcation and profound metabolic differences when compared with the diseased condition. Several metabolites such as lactate, ethanol, succinate, and glutamate were found to be of higher significance in periodontal surgery in contrast to chronic periodontitis subjects. The PLS-DA model of the studied group resulted in R2 of 0.83 and Q2 of 0.70. CONCLUSION Significant metabolites could be considered as early repair markers for chronic periodontitis disease as they are being restored to achieve symbiosis. The study, therefore, concluded the early recovery process of the diseased subjects with the restoration of possible metabolomic profile similar to the healthy controls.

中文翻译：

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牙周手术通过基于1 H NMR的代谢组学来促进唾液生物标志物的早期修复。

引言口腔微生物区系经过精心组织，可作为与口腔疾病相关的任何感染的序贯防御机制。慢性牙周炎是一种对共生和体内平衡产生微生物挑战的疾病。牙周手术是修复牙周再生过程中最有希望的方法。就早期恢复生物标志物而言，其结果令人鼓舞。目的通过1H NMR光谱法对患有慢性牙周炎的牙周手术对象的唾液进行评估，以寻找可能的早期代谢差异，以消除根除有利于共生疾病的疾病。方法本研究对1 176例人类唾液样本进行1 H NMR光谱分析，以寻找显着差异，并将其光谱数据进一步进行多变量和定量分析。结果与牙周疾病相比，牙周手术样品的1 H NMR研究显示出清晰的分界和深刻的代谢差异。与慢性牙周炎患者相比，发现几种代谢物如乳酸，乙醇，琥珀酸和谷氨酸在牙周手术中具有更高的意义。研究组的PLS-DA模型得出R2为0.83，Q2为0.70。结论重要的代谢物被认为是慢性牙周炎疾病的早期修复标志物，因为它们正在恢复以实现共生。因此，这项研究