ORANGE Represses Chloroplast Biogenesis in Etiolated Arabidopsis Cotyledons via Interaction with TCP14.,The Plant Cell

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ORANGE Represses Chloroplast Biogenesis in Etiolated Arabidopsis Cotyledons via Interaction with TCP14.
The Plant Cell ( IF 10.0 ) Pub Date : 2019-10-11 , DOI: 10.1105/tpc.18.00290
Tianhu Sun _{1,

2} , Fei Zhou ₁ , Xing-Qi Huang ₁ , Wei-Cai Chen ₁ , Meng-Juan Kong ₁ , Chang-Fang Zhou ₁ , Zhong Zhuang ₁ , Li Li ₂ , Shan Lu ₃

Affiliation

The conversion of etioplasts into chloroplasts in germinating cotyledons is a crucial transition for higher plants, enabling photoautotrophic growth upon illumination. Tight coordination of chlorophyll biosynthesis and photosynthetic complex assembly is critical for this process. ORANGE (OR), a DnaJ-like zinc finger domain-containing protein, was reported to trigger the biogenesis of carotenoid-accumulating plastids by promoting carotenoid biosynthesis and sequestration. Both nuclear and plastidic localizations of OR have been observed. Here, we show that Arabidopsis (Arabidopsis thaliana) OR physically interacts with the transcription factor TCP14 in the nucleus and represses its transactivation activity. Through this interaction, the nucleus-localized OR negatively regulates expression of EARLY LIGHT-INDUCIBLE PROTEINS (ELIPs), reduces chlorophyll biosynthesis, and delays development of thylakoid membranes in the plastids of germinating cotyledons. Nuclear abundance of OR decreased upon illumination. Together with an accumulation of TCP14 in the nucleus, this derepresses chloroplast biogenesis during de-etiolation. TCP14 is epistatic to OR and expression of ELIPs is directly regulated by the binding of TCP14 to Up1 elements in the ELIP promoter regions. Our results demonstrate that the interaction between OR and TCP14 in the nucleus leads to repression of chloroplast biogenesis in etiolated seedlings and provide new insights into the regulation of early chloroplast development.plantcell;31/12/2996/FX1F1fx1.

中文翻译：

ORANGE 通过与 TCP14 相互作用抑制黄化拟南芥子叶中的叶绿体生物发生。

发芽子叶中黄质体向叶绿体的转化是高等植物的关键转变，使得光照下能够进行光自养生长。叶绿素生物合成和光合复合物组装的紧密协调对于这一过程至关重要。据报道，ORANGE (OR) 是一种类似 DnaJ 的锌指结构域蛋白，可通过促进类胡萝卜素生物合成和隔离来触发类胡萝卜素积累质体的生物发生。 OR 的核定位和质体定位均已被观察到。在这里，我们表明拟南芥（Arabidopsis thaliana）OR与细胞核中的转录因子TCP14发生物理相互作用并抑制其反式激活活性。通过这种相互作用，核定位的 OR 负向调节早期光诱导蛋白 (ELIP) 的表达，减少叶绿素生物合成，并延迟萌发子叶质体中类囊体膜的发育。 OR 的核丰度在光照下降低。加上 TCP14 在细胞核中的积累，这会抑制去黄化过程中叶绿体的生物发生。 TCP14 对 OR 具有上位性，ELIP 的表达直接通过 TCP14 与 ELIP 启动子区域中的 Up1 元件的结合来调节。我们的结果表明，OR 和 TCP14 在细胞核中的相互作用导致黄化幼苗中叶绿体生物发生的抑制，并为早期叶绿体发育的调节提供了新的见解。plantcell;31/12/2996/FX1F1fx1。

更新日期：2019-12-11

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