Molecular assembly systems that have autonomous reproduction and Darwinian evolution abilities can be considered as minimal cell-like systems. Here we demonstrate the reproduction of cell-sized vesicles composed of AOT, i.e., sodium bis-(2-ethylhexyl) sulfosuccinate, coupled with an enzymatic polymerisation reaction occurring on the surface of the vesicles. The particular reaction used is the horseradish peroxidase-catalysed polymerisation of aniline with hydrogen peroxide as oxidant, which yields polyaniline in its emeraldine salt form (PANI-ES). If AOT micelles are added during this polymerisation reaction, the AOT - PANI-ES vesicles interact with the AOT molecules in the external solution and selectively incorporate them in their membrane, which leads to a growth of the vesicles. If the AOT vesicles also contain cholesterol, the vesicles not only show growth, but also reproduction. An important characteristic of this reproduction system is that the AOT-based vesicles encourage the synthesis of PANI-ES and PANI-ES promotes the growth of AOT vesicles.

具有自主繁殖和达尔文进化能力的分子组装系统可以被认为是最小的类细胞系统。在这里，我们证明了由AOT（即双-（2-乙基己基）磺基琥珀酸钠）组成的细胞大小囊泡的繁殖，以及在囊泡表面发生的酶促聚合反应。所使用的特定反应是辣根过氧化物酶催化的苯胺与过氧化氢作为氧化剂的聚合反应，生成的翡翠盐形式的聚苯胺（PANI-ES）。如果在该聚合反应过程中添加AOT胶束，则AOT-PANI-ES囊泡会与外部溶液中的AOT分子发生相互作用，并选择性地将其掺入其膜中，从而导致囊泡生长。如果AOT囊泡中也含有胆固醇，囊泡不仅显示出生长，而且还显示出繁殖。该生殖系统的重要特征是基于AOT的囊泡促进了PANI-ES的合成，而PANI-ES则促进了AOT囊泡的生长。